caa a22 4500 475764390 CHVBK 20180406123600.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s003960000353 doi (NATIONALLICENCE)springer-10.1007/s003960000353 Conformational changes of lysozymes with different numbers of disulfide bridges in sodium dodecyl sulfate solutions [Elektronische Daten] [Y. Moriyama, K. Hirao, K. Takeda] Four disulfide bridges of hen egg-white lysozyme were selectively reduced to obtain its derivatives with three, two, and zero disulfide bridges (designated as 3SS, 2SS, and 0SS lysozymes, respectively). The 3SS lysozyme maintained the native conformation at pH 7.0 and 3.0. Even upon the reduction of two disulfide bridges, the protein conformation still remained unchanged at pH 7.0. Upon the reduction of all four disulfide bridges, the helicity, [θ]270, and tryptophan fluorescence changed at pH 3.0 as well as at pH 7.0. The helicity of each derivative increased in a solution of sodium dodecyl sulfate (SDS). The SDS-induced helicity of the 0SS lysozyme was lower at pH 7.0 and higher at pH 3.0 than that of the intact lysozyme with four disulfide bridges. The helix formation appears to occur in originally nonhelical parts in each derivative at pH 7.0. In the cases of the 2SS and 0SS lysozymes at pH 3.0, however, some of the helices appear to be reformed also at moieties where the original helices are disrupted upon the cleavage of disulfide bridges. Springer-Verlag Berlin Heidelberg, 2000 Key words Lysozyme nationallicence Sodium dodecyl sulfate nationallicence Selective disulfide bond cleavage nationallicence Secondary structure nationallicence Fluorescence nationallicence Moriyama Y. Department of Applied Chemistry Okayama University of Science 1-1 Ridai-cho Okayama 700-0005 Japan e-mail: takeda@dac.ous.ac.jp Tel.: +81-086-2569553 Fax: +81-086-2569553, JP aut Hirao K. Department of Applied Chemistry Okayama University of Science 1-1 Ridai-cho Okayama 700-0005 Japan e-mail: takeda@dac.ous.ac.jp Tel.: +81-086-2569553 Fax: +81-086-2569553, JP aut Takeda K. Department of Applied Chemistry Okayama University of Science 1-1 Ridai-cho Okayama 700-0005 Japan e-mail: takeda@dac.ous.ac.jp Tel.: +81-086-2569553 Fax: +81-086-2569553, JP aut https://doi.org/10.1007/s003960000353 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960000353 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moriyama Y. Department of Applied Chemistry Okayama University of Science 1-1 Ridai-cho Okayama 700-0005 Japan e-mail: takeda@dac.ous.ac.jp Tel.: +81-086-2569553 Fax: +81-086-2569553, JP aut NATIONALLICENCE 700 1- Hirao K. Department of Applied Chemistry Okayama University of Science 1-1 Ridai-cho Okayama 700-0005 Japan e-mail: takeda@dac.ous.ac.jp Tel.: +81-086-2569553 Fax: +81-086-2569553, JP aut NATIONALLICENCE 700 1- Takeda K. Department of Applied Chemistry Okayama University of Science 1-1 Ridai-cho Okayama 700-0005 Japan e-mail: takeda@dac.ous.ac.jp Tel.: +81-086-2569553 Fax: +81-086-2569553, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer