caa a22 4500 475764625 CHVBK 20180406123601.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s003960000300 doi (NATIONALLICENCE)springer-10.1007/s003960000300 Enzymatic hydrolysis by Humicola lanuginosa lipase of poly(lactic acid)-poly(glycolic acid) monolayers [Elektronische Daten] [Tz. Ivanova, A. Svendsen, R. Verger, I. Panaiotov]  The enzymatic hydrolysis by Humicola lanuginosa lipase (HLL) of spread insoluble monolayers of poly (α-hydroxy acid)s with various molecular weights and various lactic-glycolic molar ratios was studied using a barostat surface balance. The interfacial hydrolysis under enzyme action leads to the progressive fragmentation of the polymer molecules. The appearance at the interface of charged insoluble fragments was detected by measuring the surface potential, while the solubilization of the small soluble fragments was detected by measuring the decrease in the surface area. The data obtained were used to test the mode of fragmentation: either random or chain-end scission. The catalytic specific activity of HLL was estimated in the framework of the random scission model and compared with the activities obtained for the hydrolysis of simple molecules of di- and tri-glycerides organized as monolayers or emulsion. Springer-Verlag Berlin Heidelberg, 2000 Key words Poly(lactic acid) nationallicence Poly (glycolic acid) nationallicence Spread monolayer nationallicence Enzymatic hydrolysis nationallicence Humicola lanuginosa lipase nationallicence Ivanova Tz Biophysical Chemistry Laboratory University of Sofia J. Bourchier str 1, 1126 Sofia, Bulgaria, BG aut Svendsen A. Novo Nordisk, Enzyme Design Novo Alle, 2880 Bagsvaerd, Denmark, DK aut Verger R. ERS 26 "Lypolyse enzymatique” du Centre National de la Recherche Scientifique B.P.71, 13402 Marseille Cedex 9, France, FR aut Panaiotov I. Biophysical Chemistry Laboratory University of Sofia J. Bourchier str 1, 1126 Sofia, Bulgaria, BG aut https://doi.org/10.1007/s003960000300 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s003960000300 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ivanova Tz Biophysical Chemistry Laboratory University of Sofia J. Bourchier str 1, 1126 Sofia, Bulgaria, BG aut NATIONALLICENCE 700 1- Svendsen A. Novo Nordisk, Enzyme Design Novo Alle, 2880 Bagsvaerd, Denmark, DK aut NATIONALLICENCE 700 1- Verger R. ERS 26 "Lypolyse enzymatique” du Centre National de la Recherche Scientifique B.P.71, 13402 Marseille Cedex 9, France, FR aut NATIONALLICENCE 700 1- Panaiotov I. Biophysical Chemistry Laboratory University of Sofia J. Bourchier str 1, 1126 Sofia, Bulgaria, BG aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer