caa a22 4500 475765494 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s007050050004 doi (NATIONALLICENCE)springer-10.1007/s007050050004 Molecular characterization of a satellite RNA associated with blackcurrant reversion nepovirus [Elektronische Daten] [S. Latvala-Kilby, A. Lemmetty, K. Lehto] Summary.:  A satellite RNA (satRNA) associated with blackcurrant reversion nepovirus (BRV) was isolated and its nucleotide sequence was determined from cDNA clones. BRV satRNA was 1432 nucleotides (nt) in length excluding the poly(A)-tail, and contained one open reading frame which encodes a polypeptide of 402 amino acids, with a calculated Mr of 44 220. The coding region was bordered by a 5′ leader sequence of 25 nt and a 3′-nontranslated region of 201 nt. Two in vitro translation products of approximately 45 kDa and 40 kDa were detected, indicating that two in-frame AUG codons at positions 26 and 134 may both be functional. Nucleotide sequence comparisons revealed a stretch of 865 nt that was 63% identical between BRV satRNA and the large satRNA of chicory yellow mottle nepovirus. A 5′-terminal consensus sequence and a 40 nt motif (located at positions 264-303 of BRV satRNA) were conserved between BRV satRNA and other nepoviral large satRNAs. 2000 Springer-Verlag/, Wien Latvala-Kilby S. Laboratory of Plant Physiology and Molecular Biology, University of Turku, Turku, Finland, FI aut Lemmetty A. Agricultural Research Centre, Plant Production Research/Plant Protection, Jokioinen, Finland, FI aut Lehto K. Laboratory of Plant Physiology and Molecular Biology, University of Turku, Turku, Finland, FI aut https://doi.org/10.1007/s007050050004 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050004 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Latvala-Kilby S. Laboratory of Plant Physiology and Molecular Biology, University of Turku, Turku, Finland, FI aut NATIONALLICENCE 700 1- Lemmetty A. Agricultural Research Centre, Plant Production Research/Plant Protection, Jokioinen, Finland, FI aut NATIONALLICENCE 700 1- Lehto K. Laboratory of Plant Physiology and Molecular Biology, University of Turku, Turku, Finland, FI aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer