caa a22 4500 475765508 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s007050050005 doi (NATIONALLICENCE)springer-10.1007/s007050050005 TT virus (TTV) genotypes in native and non-native prostitutes of Irian Jaya, Indonesia: implication for non-occupational transmission [Elektronische Daten] [Mulyanto,, M. Hijikata, M. Matsushita, G. Ingkokusmo, A. Widjaya, D. Sumarsidi, K. Kanai, Y. Ohta, S. Mishiro] Summary.:  We investigated prostitutes in Irian Jaya, an Indonesian territory of New Guinea, to know whether TT virus (TTV) is sexually transmitted and what genotypes of TTV exist there. An ORF2 region of the TTV genome was analyzed for 44 isolates from prostitutes (19 were Irian Jaya natives but 25 were immigrants from Java or other islands of Indonesia) and 26 isolates from women of child-bearing age in Surabaya as a control. The WX7HX3CX1CX5H motif sequence of the ORF2 was compared across the 70 Indonesian isolates with a reference of 47 Japanese isolates (filed in databases) and the prototype TTV isolate TA278. A total of 77 different sequences were generated from the comparison, but a phylogenetic analysis suggested that they could be divided into three categories: group A, group B and others (the third group was highly diverse). Interestingly, most of the Indonesian isolates belonged to group B (74%): this rate was considerably higher than that observed previously in Japanese isolates. Group B isolates were further compared for the N-terminal 95 amino acids of the ORF2, with a result that the natives of Irian Jaya had a different pattern of genotype distribution from other groups. In particular, 9 out of 19 isolates from the Irian Jaya-natives were co-classified under a distinct branch, to which none of the other Indonesian and Japanese isolates belonged. Our data indicate that TTV genotypes reflect the birth place of the infected prostitutes rather than their work environment, and thus suggest that an infection is more likely during the early period of life than through sexual transmission. In addition, the presence of an Irian Jaya-specific genotype is intriguing from an anthropological and viral evolutionary point of view, because Irian Jaya has been isolated from contact with other areas for a long time. 2000 Springer-Verlag/, Wien Mulyanto, West Nusa Tenggara Hepatitis Laboratory, Mataram, Indonesia, ID aut Hijikata M. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut Matsushita M. The Fourth Department of Medicine, Teikyo University School of Medicine, Kanagawa, Japan, JP aut Ingkokusmo G. Irian Jaya Provincial Health Office, Jaya Pura, Indonesia, ID aut Widjaya A. Program for Appropriate Technology in Health, Jakarta, Indonesia, ID aut Sumarsidi D. West Nusa Tenggara Hepatitis Laboratory, Mataram, Indonesia, ID aut Kanai K. Department of Gastroenterology, Toshiba General Hospital, Tokyo, Japan, JP aut Ohta Y. Department of Gastroenterology, Toshiba General Hospital, Tokyo, Japan, JP aut Mishiro S. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut https://doi.org/10.1007/s007050050005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mulyanto West Nusa Tenggara Hepatitis Laboratory, Mataram, Indonesia, ID aut NATIONALLICENCE 700 1- Hijikata M. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Matsushita M. The Fourth Department of Medicine, Teikyo University School of Medicine, Kanagawa, Japan, JP aut NATIONALLICENCE 700 1- Ingkokusmo G. Irian Jaya Provincial Health Office, Jaya Pura, Indonesia, ID aut NATIONALLICENCE 700 1- Widjaya A. Program for Appropriate Technology in Health, Jakarta, Indonesia, ID aut NATIONALLICENCE 700 1- Sumarsidi D. West Nusa Tenggara Hepatitis Laboratory, Mataram, Indonesia, ID aut NATIONALLICENCE 700 1- Kanai K. Department of Gastroenterology, Toshiba General Hospital, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Ohta Y. Department of Gastroenterology, Toshiba General Hospital, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Mishiro S. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer