caa a22 4500 475765532 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s007050050011 doi (NATIONALLICENCE)springer-10.1007/s007050050011 Genetic and antigenic variance of foot-and-mouth disease virus type Asia1 [Elektronische Daten] [O. Marquardt, M. M. Rahman, B. Freiberg] Summary.:  The capsid protein encoding genes of five recent type Asia1 foot-and-mouth disease virus isolates, representative of three genotypes, were sequenced. The deduced amino acid sequences were aligned to each other and to two published sequences. The sequence differences suggested different antigenic properties of the isolates. One isolate was used to generate monoclonal antibodies (mAbs) which were analyzed for neutralizing activity and reactivity with trypsinized virus. Trypsin removes the major antigenic sites located at VP1. The five virus isolates formed three reaction patterns with the mAbs, irrespective of their genotype. Combination of all data allowed to suggest the location of the epitope of each antibody: the VP1 G-H and the VP2 B-C loop, the VP3 B-B knob, and the N-terminus of VP2, respectively, were involved. 2000 Springer-Verlag/, Wien Marquardt O. Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tübingen, Federal Republic of Germany, DE aut Rahman M. M. Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh aut Freiberg B. Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tübingen, Federal Republic of Germany, DE aut https://doi.org/10.1007/s007050050011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marquardt O. Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tübingen, Federal Republic of Germany, DE aut NATIONALLICENCE 700 1- Rahman M. M. Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh aut NATIONALLICENCE 700 1- Freiberg B. Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tübingen, Federal Republic of Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer