caa a22 4500 475765540 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s007050050012 doi (NATIONALLICENCE)springer-10.1007/s007050050012 High frequency recombination between homologous baculoviruses in cell culture [Elektronische Daten] [J. P. Hajós, J. Pijnenburg, M. Usmany, D. Zuidema, P. Závodszky, J. M. Vlak] Summary.:  The frequency of recombination between homologous baculoviruses was investigated in cell culture upon coinfection with two Autographa californica nucleopolyhedrovirus (AcMNPV) recombinants. These parental recombinants differed at two loci, separated by 20 kb, each carrying a different marker. The progeny recombinants generated by homologous recombination were easily distinguishable by the presence or absence of these markers. The mean percentage of the newly generated recombinants relative to a single locus varied between 23 and 41%, depending on the multiplicity of infection used for coinfection. These results provide further evidence that homologous recombination occurring during baculovirus replication is a highly frequent event. 2000 Springer-Verlag/, Wien Hajós J. P. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut Pijnenburg J. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut Usmany M. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut Zuidema D. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut Závodszky P. Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary, HU aut Vlak J. M. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut https://doi.org/10.1007/s007050050012 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050012 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hajós J. P. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Pijnenburg J. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Usmany M. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Zuidema D. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Závodszky P. Institute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Hungary, HU aut NATIONALLICENCE 700 1- Vlak J. M. Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer