caa a22 4500 475765575 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s007050050007 doi (NATIONALLICENCE)springer-10.1007/s007050050007 Stability of a varicella-zoster virus glycoprotein E epitope [Elektronische Daten] [A. Vafai, B. Forghani, D. Kilpatrick, J. Ling, V. Shankar] Summary.:  The epitope stability of a varicella-zoster virus (VZV) glycoprotein E (gE) was analyzed with monoclonal antibodies (mAbs) in cells infected with different passages of various VZV strains and isolates. The gE-specific mAbs recognized same antigenic sites (epitopes) in VZV isolates with various passage history. All VZV strains and virus-isolates reacted with an anti-gE monoclonal antibody by immunoprecipitation, or indirect fluorescent antibody staining test. Sera from VZV seropositive individuals reacted with a truncated VZV gE glycoprotein, designated TgpI-511. Also, human mononuclear cells (MNCs) stimulated with TgpI-511 glycoprotein were shown to produce VZV-specific antibodies in vitro. The results demonstrated the stability of these gE epitopes tested in this study in TgpI-511 and among the VZV-isolates obtained from different passages. These results also suggest that VZV glycoproteins as well as live attenuated or killed varicella vaccines containing these epitopes could be used as therapeutic booster vaccines in adults and the elderly to prevent zoster. 2000 Springer-Verlag/, Wien Vafai A. Biologics Branch, Scientific Resources Program, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, U.S.A., US aut Forghani B. Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California State Department of Health Services, Berkeley, California, U.S.A., US aut Kilpatrick D. Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, U.S.A., US aut Ling J. Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California State Department of Health Services, Berkeley, California, U.S.A., US aut Shankar V. Biologics Branch, Scientific Resources Program, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, U.S.A., US aut https://doi.org/10.1007/s007050050007 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vafai A. Biologics Branch, Scientific Resources Program, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, U.S.A., US aut NATIONALLICENCE 700 1- Forghani B. Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California State Department of Health Services, Berkeley, California, U.S.A., US aut NATIONALLICENCE 700 1- Kilpatrick D. Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, U.S.A., US aut NATIONALLICENCE 700 1- Ling J. Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California State Department of Health Services, Berkeley, California, U.S.A., US aut NATIONALLICENCE 700 1- Shankar V. Biologics Branch, Scientific Resources Program, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer