caa a22 4500 475765656 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s007050050014 doi (NATIONALLICENCE)springer-10.1007/s007050050014 High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector [Elektronische Daten] [J. Y. Choi, S. D. Woo, H. K. Lee, H. K. Hong, Y. H. Je, J. H. Park, J. Y. Song, S. H. An, S. K. Kang] Summary.:  For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 × 103 HA units/ml from infected Bm5 cells, 2.1× 105 HA units/larvae from infected larval fat body, and 1.6× 106 HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines. 2000 Springer-Verlag/, Wien Choi J. Y. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut Woo S. D. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut Lee H. K. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut Hong H. K. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut Je Y. H. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut Park J. H. National Veterinary Research and Quarantine Service, Anyang, Korea, KR aut Song J. Y. National Veterinary Research and Quarantine Service, Anyang, Korea, KR aut An S. H. National Veterinary Research and Quarantine Service, Anyang, Korea, KR aut Kang S. K. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut https://doi.org/10.1007/s007050050014 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi J. Y. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut NATIONALLICENCE 700 1- Woo S. D. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut NATIONALLICENCE 700 1- Lee H. K. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut NATIONALLICENCE 700 1- Hong H. K. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut NATIONALLICENCE 700 1- Je Y. H. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut NATIONALLICENCE 700 1- Park J. H. National Veterinary Research and Quarantine Service, Anyang, Korea, KR aut NATIONALLICENCE 700 1- Song J. Y. National Veterinary Research and Quarantine Service, Anyang, Korea, KR aut NATIONALLICENCE 700 1- An S. H. National Veterinary Research and Quarantine Service, Anyang, Korea, KR aut NATIONALLICENCE 700 1- Kang S. K. Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea, KR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer