caa a22 4500 475765672 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s007050070120 doi (NATIONALLICENCE)springer-10.1007/s007050070120 Recombinant expression and modification analysis of protein agno-1b encoded by avian polyomavirus BFDV [Elektronische Daten] [Q. Liu, M. Hintz, J. Li, M. Linder, R. Geyer, G. Hobom] Summary.:  Among two pairs of agnoproteins encoded in upstream positions in the late mRNAs of avian polyomavirus BFDV, either agno-1a or its splice derivative agno-1b are required for viral propagation. Out of the two proteins both of which consist of multiple electrophoretic subspecies, the smaller and less complex agno-1b has been cDNA-cloned into an influenza-virus /RNA-polymerase I expression system for production of higher amounts of this protein in infected chicken embryo fibroblasts. Fractional modification of agno-1b by phosphorylation at residues serine 51, serine 53, and threonine 73 is demonstrated through dephosphorylation by alkaline phosphatase, mass spectrometry of individual protein species isolated by strong anion exchange chromatography, and single or multiple alanine substitutions of serine or threonine residues in site-directed mutagenesis. Springer-Verlag/Wien, 2000 Liu Q. Institut für Mikrobiologie und Molekularbiologie der Universität Giessen, Giessen, Federal Republic of Germany, DE aut Hintz M. Biochemisches Institut am Klinikum der Universität Giessen, Giessen, Federal Republic of Germany, DE aut Li J. Institut für Mikrobiologie und Molekularbiologie der Universität Giessen, Giessen, Federal Republic of Germany, DE aut Linder M. Biochemisches Institut am Klinikum der Universität Giessen, Giessen, Federal Republic of Germany, DE aut Geyer R. Biochemisches Institut am Klinikum der Universität Giessen, Giessen, Federal Republic of Germany, DE aut Hobom G. Institut für Mikrobiologie und Molekularbiologie der Universität Giessen, Giessen, Federal Republic of Germany, DE aut https://doi.org/10.1007/s007050070120 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070120 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Q. Institut für Mikrobiologie und Molekularbiologie der Universität Giessen, Giessen, Federal Republic of Germany, DE aut NATIONALLICENCE 700 1- Hintz M. Biochemisches Institut am Klinikum der Universität Giessen, Giessen, Federal Republic of Germany, DE aut NATIONALLICENCE 700 1- Li J. Institut für Mikrobiologie und Molekularbiologie der Universität Giessen, Giessen, Federal Republic of Germany, DE aut NATIONALLICENCE 700 1- Linder M. Biochemisches Institut am Klinikum der Universität Giessen, Giessen, Federal Republic of Germany, DE aut NATIONALLICENCE 700 1- Geyer R. Biochemisches Institut am Klinikum der Universität Giessen, Giessen, Federal Republic of Germany, DE aut NATIONALLICENCE 700 1- Hobom G. Institut für Mikrobiologie und Molekularbiologie der Universität Giessen, Giessen, Federal Republic of Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer