caa a22 4500 475765699 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s007050070117 doi (NATIONALLICENCE)springer-10.1007/s007050070117 Structure and variability of the 3′ end of RNA 3 of Beet soil-borne pomovirus - a virus with uncertain pathogenic effects [Elektronische Daten] [R. Koenig, C. W. A. Pleij, G. Büttner] Summary.:  PCR products representing c. 550 3′ terminal bases of Beet soil-borne pomovirus (BSBV) RNA 3 were compared for sourcesof this virus from all major sugarbeet-growing areas in Germany. In noneof these areas conspicious symptoms could be attributed to the presence of BSBV. Single strand conformation polymorphism analyses suggested that the BSBV genome may be very variable. This was confirmedby nucleotide sequence analysis. Each PCR product which was analysed showed sequence differences to others. Even the PCR products obtained from plants grown in the same soil sample were different. The highly variable nature of the BSBV genome is in contrast to the much more conserved nature of the Beet necrotic yellow vein virus genome. Bymeans of the STAR programme a secondary structure was predicted for the 3′ end of BSBV RNA 3, in which some areas are highly conserved, whereas others are characterized by a clustering of nucleotide exchanges. Springer-Verlag/Wien, 2000 Koenig R. Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Braunschweig, Germany, DE aut Pleij C. W. A. Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands, NL aut Büttner G. Institut für Zuckerrübenforschung, Göttingen, Germany, DE aut https://doi.org/10.1007/s007050070117 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070117 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koenig R. Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenvirologie, Mikrobiologie und biologische Sicherheit, Braunschweig, Germany, DE aut NATIONALLICENCE 700 1- Pleij C. W. A. Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands, NL aut NATIONALLICENCE 700 1- Büttner G. Institut für Zuckerrübenforschung, Göttingen, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer