caa a22 4500 475765710 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s007050070123 doi (NATIONALLICENCE)springer-10.1007/s007050070123 Specific detection of avian pneumovirus (APV) US isolates by RT-PCR [Elektronische Daten] [H. J. Shin, G. Rajashekara, F. F. Jirjis, D. P. Shaw, S. M. Goyal, D. A. Halvorson, K. V. Nagaraja] Summary.:  This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as low as 8 × 10−5 TCID50 (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota. Springer-Verlag/Wien, 2000 Shin H. J. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Rajashekara G. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Jirjis F. F. Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Shaw D. P. Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Goyal S. M. Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Halvorson D. A. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Nagaraja K. V. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut https://doi.org/10.1007/s007050070123 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070123 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shin H. J. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut NATIONALLICENCE 700 1- Rajashekara G. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut NATIONALLICENCE 700 1- Jirjis F. F. Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut NATIONALLICENCE 700 1- Shaw D. P. Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut NATIONALLICENCE 700 1- Goyal S. M. Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut NATIONALLICENCE 700 1- Halvorson D. A. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut NATIONALLICENCE 700 1- Nagaraja K. V. Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer