caa a22 4500 475765729 CHVBK 20180406123604.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s007050070119 doi (NATIONALLICENCE)springer-10.1007/s007050070119 Antigenic and genetic characterization of the fusion (F) protein of mumps virus strains [Elektronische Daten] [T. Tecle, B. Johansson, Z. Yun, C. Örvell] Summary.:  Twelve strains of mumps virus, belonging to the A, C and D genotypes of the small hydrophobic (SH) protein gene, were investigated by nucleotide sequencing of the fusion protein gene. The nucleotide sequences and deduced amino acid sequences were aligned and compared with previously reported sequences of the gene. In addition an antigenic comparison between the F protein of different strains of the A, C and D genotypes was performed with ten monoclonal antibodies directed against the F protein of genotype A. Phylogenetic analysis of the coding region of the F gene showed the expected clustering of the different genotypes, as previously determined from the SH protein gene. Comparison of the 538 long amino acid sequence of the protein showed that only a small number of amino acids differed between the viral strains. The A genotype differed from B, C and D whereas the latter showed fewer consistent amino acid differences between themselves. Nine of the ten monoclonal antibodies reacted with the C and D genotypes and one failed to react with these genotypes. It is concluded that the structure and antigenicity of the F protein is well conserved both intra- and intergenotypically over long periods of time. Springer-Verlag/Wien, 2000 Tecle T. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut Johansson B. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut Yun Z. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut Örvell C. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut https://doi.org/10.1007/s007050070119 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070119 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tecle T. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut NATIONALLICENCE 700 1- Johansson B. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut NATIONALLICENCE 700 1- Yun Z. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut NATIONALLICENCE 700 1- Örvell C. Huddinge University Hospital, Department of Clinical Virology, Karolinska Institute, Huddinge, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer