caa a22 4500 475765834 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s007050070115 doi (NATIONALLICENCE)springer-10.1007/s007050070115 Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype [Elektronische Daten] [R. Allende, G. F. Kutish, W. Laegreid, Z. Lu, T. L. Lewis, D. L. Rock, J. Friesen, J. A. Galeota, A. R. Doster, F. A. Osorio] Summary.:  Although live-attenuated vaccines have been used for some time to control clinical symptoms of the porcine reproductive and respiratory syndrome (PRRS), the molecular bases for the attenuated phenotype remain unclear. We had previously determined the genomic sequence of the pathogenic PRRSV 16244B. Limited comparisons of the structural protein coding sequence of an attenuated vaccine strain have shown 98% homology to the pathogenic 16244B. Here we have confirmed the attenuated phenotype and determined the genomic sequence of that attenuated PRRSV vaccine and compared it to its parental VR-2332 and the 16244B strains. The attenuated vaccine sequence was colinear with that of the strain 16244B sequence containing no gaps and 212 substitutions over 15,374 determined nucleotide sequence. We identified nine amino acid changes distributed in Nsp1β, Nsp2, Nsp10, ORF2, ORF3, ORF5 and ORF6. These changes may provide the molecular bases for the observed attenuated phenotype. Springer-Verlag/Wien, 2000 Allende R. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut Kutish G. F. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut Laegreid W. U.S. Meat Animal Research Center, USDA/ARS, Clay Center, Nebraska, U.S.A., US aut Lu Z. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut Lewis T. L. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut Rock D. L. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut Friesen J. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut Galeota J. A. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut Doster A. R. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut Osorio F. A. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut https://doi.org/10.1007/s007050070115 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070115 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Allende R. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Kutish G. F. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut NATIONALLICENCE 700 1- Laegreid W. U.S. Meat Animal Research Center, USDA/ARS, Clay Center, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Lu Z. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut NATIONALLICENCE 700 1- Lewis T. L. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut NATIONALLICENCE 700 1- Rock D. L. Plum Island Animal Disease Center, USDA/ARS, Greenport, New York, U.S.A., US aut NATIONALLICENCE 700 1- Friesen J. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Galeota J. A. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Doster A. R. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Osorio F. A. Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer