caa a22 4500 475765842 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070056 doi (NATIONALLICENCE)springer-10.1007/s007050070056 Comparative sequence analysis identified mutations outside the NSP4 cytotoxic domain of tissue culture-adapted ATCC-Wa strain of human rotavirus and a novel inter-species variable domain in its C-terminus [Elektronische Daten] [K. V. Krishna Mohan, C. D. Atreya] Summary.:  Complete nucleotide sequence of the tissue culture-adapted ATCC*-Wa strain of human rotavirus NSP4 was determined. Sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (A or G) in the coding sequence (NSP4-A or NSP4-G) leading to a change from neutral glutamine97 in NSP4-A to a positively charged arginine97 in NSP4-G originating from the same ATCC-Wa preparation. In addition to this, both forms of ATCC-Wa NSP4 revealed three mutations at nucleotide positions 88 (T to C), 142 (C to T) and 572 (G to A), when compared to the previously reported NSP4 sequence from virulent Wa strain. The former two mutations were in the coding sequence and resulted in a leucine16 to serine16 and a proline34 to leucine34 change, while the third mutation was in the 3′ non-coding region of the gene. The two amino acid changes may contribute to the ‘tissue culture-adaptation' of ATCC-Wa strain. The ATCC-Wa NSP4 sequence was found to differ from the previously reported NSP4 sequence of attenuated Wa strain by lacking a mutation at 133 (T to C), though the mutations at 88 and 142 were present in both strains. Furthermore, comparison of deduced amino acid sequence of NSP4 from human, bovine, porcine and simian rotavirus strains identified a seven-residue (positions 135-141) inter-species variable domain in its C-terminal region. Springer-Verlag/Wien, 2000 Krishna Mohan K. V. Laboratory of Pediatric and Respiratory Viral Diseases, Section of Viral Pathogenesis and Adverse Reactions, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, U.S.A., US aut Atreya C. D. Laboratory of Pediatric and Respiratory Viral Diseases, Section of Viral Pathogenesis and Adverse Reactions, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, U.S.A., US aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1789-1799 0304-8608 145:9<1789 2000 145 705 https://doi.org/10.1007/s007050070056 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070056 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Krishna Mohan K. V. Laboratory of Pediatric and Respiratory Viral Diseases, Section of Viral Pathogenesis and Adverse Reactions, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, U.S.A., US aut NATIONALLICENCE 700 1- Atreya C. D. Laboratory of Pediatric and Respiratory Viral Diseases, Section of Viral Pathogenesis and Adverse Reactions, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, U.S.A., US aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1789-1799 0304-8608 145:9<1789 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer