caa a22 4500 475765850 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070059 doi (NATIONALLICENCE)springer-10.1007/s007050070059 Genome replication of Newcastle disease virus: involvement of the rule-of-six [Elektronische Daten] [B. P. H. Peeters, Y. K. Gruijthuijsen, O. S. de Leeuw, A. L. J. Gielkens] Summary.:  We examined replication of Newcastle disease virus (NDV) by using minigenomes consisting of the 3′ leader and 5′ trailer regions of NDV flanking a reporter gene encoding secreted placental alkaline phosphatase (SEAP). Negative-sense minigenome RNA was generated from transfected plasmid DNA by means of in vivo transcription. Subsequent replication of minigenome RNA was determined either after infection with NDV helpervirus or after contransfection with helperplasmids that expressed the essential viral replication proteins NP, P, and L. In both systems, efficient replication of minigenome RNA was observed only if the genome size was a multiple of six nucleotides. Hence, in these systems, replication of NDV minigenome RNA's is strictly dependent on the rule-of-six. When the supernatant from helpervirus-infected, transfected cells was used to infect fresh monolayers, efficient transfer of SEAP activity by virus-like particles was observed only if the size of the minigenome RNA obeyed the rule-of-six. However, after several serial passages, we also observed efficient transfer of SEAP activity by virus-like particles derived from minigenome RNA's that did not obey the rule-of-six. Evidence was obtained which indicated that successful replication of these minigenomes was not due to a change in genome size. Springer-Verlag/Wien, 2000 Peeters B. P. H. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut Gruijthuijsen Y. K. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut de Leeuw O. S. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut Gielkens A. L. J. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1829-1845 0304-8608 145:9<1829 2000 145 705 https://doi.org/10.1007/s007050070059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peeters B. P. H. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Gruijthuijsen Y. K. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- de Leeuw O. S. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Gielkens A. L. J. Institute for Animal Science and Health (ID-Lelystad), Lelystad, The Netherlands, NL aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1829-1845 0304-8608 145:9<1829 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer