caa a22 4500 475765869 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070063 doi (NATIONALLICENCE)springer-10.1007/s007050070063 Detection of herpesviral sequences in tissues of green turtles with fibropapilloma by polymerase chain reaction [Elektronische Daten] [Y. Lu, Y. Wang, Q. Yu, A. A. Aguirre, G. H. Balazs, V. R. Nerurkar, R. Yanagihara] Summary.:  An alpha-herpesvirus has been associated recently with green turtle fibropapilloma (FP). To further clarify the role of this newfound green turtle herpesvirus (GTHV) in the pathogenesis of FP, various normal-appearing tissues and organs (including skin, eye, brain, heart, liver, spleen, intestine, lung, kidney, nerve, gonad, tongue, gall bladder, urinary bladder, thyroid and peripheral blood mononuclear cells (PBMC) from blood) and tumor tissues from 19 green turtles (Chelonia mydas) with FP, and tissues from three green turtles without FP, collected during 1997 to 1999 in the Hawaiian Islands, were tested for GTHV sequences by nested polymerase chain reaction (PCR), using GTHV-specific oligonuclotide primers. GTHV sequences were detected in all tumors (51/51) and most tissues (133/167) of tumored turtles. By contrast, such sequences were undetectable in tissues (0/28) of three non-tumored turtles. Analysis of GTHV sequences detected in different tissues and tumors revealed a low degree of genetic diversity (<1%). The wide distribution of this newfound herpesvirus in tumors and tissues of tumored green turtles and its absence in tissues of non-tumored turtles, argues for an etiologic role in FP. Springer-Verlag/Wien, 2000 Lu Y. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut Wang Y. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut Yu Q. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut Aguirre A. A. Wildlife Preservation Trust International/Center for Conservation Medicine, Tufts School of Veterinary Medicine, North Grafton, Massachusetts, U.S.A., US aut Balazs G. H. National Marine Fisheries Services, Southwest Fisheries Science Center, Honolulu Laboratory, Honolulu, Hawaii, U.S.A., US aut Nerurkar V. R. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut Yanagihara R. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1885-1893 0304-8608 145:9<1885 2000 145 705 https://doi.org/10.1007/s007050070063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Y. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut NATIONALLICENCE 700 1- Wang Y. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut NATIONALLICENCE 700 1- Yu Q. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut NATIONALLICENCE 700 1- Aguirre A. A. Wildlife Preservation Trust International/Center for Conservation Medicine, Tufts School of Veterinary Medicine, North Grafton, Massachusetts, U.S.A., US aut NATIONALLICENCE 700 1- Balazs G. H. National Marine Fisheries Services, Southwest Fisheries Science Center, Honolulu Laboratory, Honolulu, Hawaii, U.S.A., US aut NATIONALLICENCE 700 1- Nerurkar V. R. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut NATIONALLICENCE 700 1- Yanagihara R. Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Leahi Hospital, Honolulu, Hawaii, U.S.A., US aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1885-1893 0304-8608 145:9<1885 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer