caa a22 4500 475765885 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070055 doi (NATIONALLICENCE)springer-10.1007/s007050070055 Prevalence of equine herpesvirus type 1 latency detected by polymerase chain reaction [Elektronische Daten] [R. Carvalho, A. M. Oliveira, A. M. Souza, L. M. F. Passos, A. S. Martins] Summary.:  In this study, an improved polymerase chain reaction (PCR) was used for detection of DNA of latent EHV-1 strains from several sources. Three pairs of oligonucleotide primers spanning fragments of 333 bp, 226 bp and 268 bp of the thymidine kinase (tk) gene, and one primer pair spanning 225 bp of the glycoprotein C (gC) gene were used in specific amplifications. Primers for EHV-4 PCR were also designed. Restriction digests with TaqI confirmed the identity of tk PCR fragments from EHV-1. The sensitivity to detect PCR products was further improved by visualisation in silver-stained acrylamide gels. PCR assays were applied to 267 samples including pools of tissue, peripheral blood leukocytes (PBL) and nasal swabs of archived, farms and abattoir specimens from a total of 116 animals. The EHV-1 DNA was found in 88% of the analysed samples. The prevalence of the EHV-1 latent or persistent form in adult horses was similar to others reports but found higher than previously described in foetuses and young foals. EHV-4 latency was not detected in the Brazilian studied specimens. Springer-Verlag/Wien, 2000 Carvalho R. Ministério da Agricultura e do Abastecimento, Belo Horizonte, MG, Brazil, BR aut Oliveira A. M. Ministério da Agricultura e do Abastecimento, Belo Horizonte, MG, Brazil, BR aut Souza A. M. Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil, BR aut Passos L. M. F. Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil, BR aut Martins A. S. Instituto de Ciências Biológicas - UFMG, Belo Horizonte, MG, Brazil, BR aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1773-1787 0304-8608 145:9<1773 2000 145 705 https://doi.org/10.1007/s007050070055 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070055 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carvalho R. Ministério da Agricultura e do Abastecimento, Belo Horizonte, MG, Brazil, BR aut NATIONALLICENCE 700 1- Oliveira A. M. Ministério da Agricultura e do Abastecimento, Belo Horizonte, MG, Brazil, BR aut NATIONALLICENCE 700 1- Souza A. M. Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil, BR aut NATIONALLICENCE 700 1- Passos L. M. F. Escola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil, BR aut NATIONALLICENCE 700 1- Martins A. S. Instituto de Ciências Biológicas - UFMG, Belo Horizonte, MG, Brazil, BR aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1773-1787 0304-8608 145:9<1773 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer