caa a22 4500 475765907 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070060 doi (NATIONALLICENCE)springer-10.1007/s007050070060 Human astrovirus isolation and propagation in multiple cell lines [Elektronische Daten] [J. P. Brinker, N. R. Blacklow, J. E. Herrmann] Summary.:  Laboratory adapted human astrovirus serotypes 1 through 7 were tested for growth in 15 human, 7 simian, and 10 other non-primate mammalian cell lines. Propagation of all seven serotypes was successful in the human cell lines Caco-2, T84, HT-29, and in the African green monkey kidney cell line MA-104. Both primary and secondary African green monkey kidney cells were more effective than Rhesus monkey kidney cells for cultivation of astrovirus. Except for human foreskin cells, all of the other human and simian cell lines supported growth of at least one astrovirus serotype. The only non-primate cell line that permitted sustained passage of astroviruses was the BHK-21 (C13) cell line for astrovirus serotype 2. Seventeen human stool specimens that had previously been shown to be astrovirus positive by ELISA were cultured in Caco-2, T84, HT-29, SK-CO-1, PLC/PRF/5, MA-104, and VERO cells. Caco-2 cells (13 isolates), T84 cells (12 isolates) and PLC/PRF/5 cells (12 isolates) were the cell lines most effective for isolation of human astroviruses from clinical stool specimens. By immunofluorescent staining of infected cells, culturing of the same 17 specimens in shell vials for 18 h was positive for astroviruses in all 17 specimens in Caco-2 cells, 12 in T84 cells, and 7 in PLC/PRF/5 cells. Shell vial assay is suitable as a rapid and sensitive culture technique for detection of astroviruses in clinical specimens. Springer-Verlag/Wien, 2000 Brinker J. P. Division of Infectious Diseases, University of Massachusetts Medical School, Worcester, Massachusetts, U.S.A., US aut Blacklow N. R. Division of Infectious Diseases, University of Massachusetts Medical School, Worcester, Massachusetts, U.S.A., US aut Herrmann J. E. Division of Infectious Diseases, University of Massachusetts Medical School, Worcester, Massachusetts, U.S.A., US aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1847-1856 0304-8608 145:9<1847 2000 145 705 https://doi.org/10.1007/s007050070060 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070060 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brinker J. P. Division of Infectious Diseases, University of Massachusetts Medical School, Worcester, Massachusetts, U.S.A., US aut NATIONALLICENCE 700 1- Blacklow N. R. Division of Infectious Diseases, University of Massachusetts Medical School, Worcester, Massachusetts, U.S.A., US aut NATIONALLICENCE 700 1- Herrmann J. E. Division of Infectious Diseases, University of Massachusetts Medical School, Worcester, Massachusetts, U.S.A., US aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1847-1856 0304-8608 145:9<1847 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer