caa a22 4500 475765915 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070054 doi (NATIONALLICENCE)springer-10.1007/s007050070054 Baculovirus infection blocks the progression of fat body degradation during metamorphosis in Bombyx mori [Elektronische Daten] [M. Iwanaga, W. K. Kang, M. Kobayashi, S. Maeda] Summary.:  The effect of baculovirus infection into silkworm pupa particularly on programmed fat body degradation during metamorphosis was investigated. Pupal fat body degradation did not occur following infection with Bombyx mori nucleo-polyhedrovirus (BmNPV). There were no histolytic differences between the fat body tissues of mock and BmNPV infected papae until 48 h postinfection (p.i.). Between 48 and 72 h p.i., significant differences were observed. In order to determine whether the histolysis of fat body was due to apoptosis, genomic DNAs were purified at various p.i. times and analyzed. Rapid genomic DNA fragmentation was observed at 24 and 48 h after pupation in both mock and BmNPV infected pupae. However, BmNPV infection clearly inhibited DNA fragmentation and ladder formation at 72 h and later times p.i. Furthermore, pulse-labeling analysis showed that BmNPV infection restored protein synthesis in fat body cells. These results suggested that fat body degradation during pupal-adult development was due to apoptosis, and that BmNPV was able to evoke a cellular response in these cells. Springer-Verlag/Wien, 2000 Iwanaga M. Laboratory of Molecular Entomology and Baculovirology, RIKEN, Wako, Japan, JP aut Kang W. K. Laboratory of Molecular Entomology and Baculovirology, RIKEN, Wako, Japan, JP aut Kobayashi M. Laboratory of Insect Genetics and Bioscience, Department of Agricultural Science, The University of Tokyo, Tokyo, Japan, JP aut Maeda S. Laboratory of Insect Genetics and Bioscience, Department of Agricultural Science, The University of Tokyo, Tokyo, Japan, JP aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1763-1771 0304-8608 145:9<1763 2000 145 705 https://doi.org/10.1007/s007050070054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwanaga M. Laboratory of Molecular Entomology and Baculovirology, RIKEN, Wako, Japan, JP aut NATIONALLICENCE 700 1- Kang W. K. Laboratory of Molecular Entomology and Baculovirology, RIKEN, Wako, Japan, JP aut NATIONALLICENCE 700 1- Kobayashi M. Laboratory of Insect Genetics and Bioscience, Department of Agricultural Science, The University of Tokyo, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Maeda S. Laboratory of Insect Genetics and Bioscience, Department of Agricultural Science, The University of Tokyo, Tokyo, Japan, JP aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1763-1771 0304-8608 145:9<1763 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer