caa a22 4500 475765923 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070068 doi (NATIONALLICENCE)springer-10.1007/s007050070068 Mapping of functional domains on the influenza A virus RNA polymerase PB2 molecule using monoclonal antibodies [Elektronische Daten] [M. Hatta, Y. Asano, K. Masunaga, T. Ito, K. Okazaki, T. Toyoda, Y. Kawaoka, A. Ishihama, H. Kida] Summary.:  Monoclonal antibodies against the PB2 of A/Puerto Rico/8/34 (A/PR/ 8/34) (H1N1) were prepared in order to define the functional domains of the RNA polymerase of influenza virus. The fifteen monoclonal antibodies that were generated were divided into 4 groups on the basis of ELISA binding to PB2 or its peptide fragments. Six Group I antibodies that bound to the PB2 N-terminal region (amino acids 1-104) did not inhibit transcription by the viral ribonucleoprotein complex. A single Group II antibody recognizing the region of amino acids 206-259 inhibited ApG-primed transcription. Groups III and IV antibodies bound to the C-terminal region of amino acids 660-759. Of these, Group III antibodies inhibited transcription. The present results identify multiple monoclonal antibody binding domains in PB2, two of which, when bound by antibodies, negatively affect viral RNA transcription. Springer-Verlag/Wien, 2000 Hatta M. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Asano Y. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut Masunaga K. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut Ito T. Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Tottori, Japan, JP aut Okazaki K. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Toyoda T. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut Kawaoka Y. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, U.S.A., US aut Ishihama A. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut Kida H. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1947-1961 0304-8608 145:9<1947 2000 145 705 https://doi.org/10.1007/s007050070068 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070068 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hatta M. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Asano Y. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut NATIONALLICENCE 700 1- Masunaga K. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Ito T. Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Tottori, Japan, JP aut NATIONALLICENCE 700 1- Okazaki K. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Toyoda T. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Kawaoka Y. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, U.S.A., US aut NATIONALLICENCE 700 1- Ishihama A. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut NATIONALLICENCE 700 1- Kida H. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1947-1961 0304-8608 145:9<1947 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer