caa a22 4500 47576594X CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070064 doi (NATIONALLICENCE)springer-10.1007/s007050070064 Indian citrus ringspot virus: a proposed new species with some affinities to potex-, carla-, fovea- and allexiviruses [Elektronische Daten] [G. Rustici, G. P. Accotto, E. Noris, V. Masenga, E. Luisoni, R. G. Milne] Summary.:  An isolate of Indian citrus ringspot virus from Kinnow mandarin in northern India had flexuous particles with evident cross-banding and a modal length of 650 nm. It was mechanically transmitted to five herbaceous hosts including Phaseolus vulgaris cv Saxa, in which it became systemic. In thin sections, virus particles were observed in the cytoplasm of parenchyma cells but no specific inclusions were seen. The virus was purified from infected Saxa bean leaves and an antiserum prepared. There was no serological cross-reaction with representative allexi-, capillo-, potex- and trichoviruses, except a faint one-way reaction with Potato virus X. Purified virus yielded a major band, the presumed coat protein (CP), of about 34 kDa, and a single ssRNA of about 7.5 kb, which was infectious. Two ORFs encoding putative proteins of 34 kDa and 23 kDa were located in the 3′ part of the RNA. The product of the 34 kDa ORF was confirmed as the CP by expression in E. coli. The derived amino acid sequence of the CP contained some short motifs similar to those of potex-, fovea-, carla- and allexiviruses but otherwise there was no strong similarity to any of these. The 23 kDa ORF contained a zinc finger-like sequence, as in similar ORFs in carla- and allexiviruses but overall amino acid homology with these was low. The virus does not appear to fall into any known genus. A new species is proposed. Serological and molecular diagnostic reagents were prepared. Springer-Verlag/Wien, 2000 Rustici G. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut Accotto G. P. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut Noris E. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut Masenga V. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut Luisoni E. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut Milne R. G. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1895-1908 0304-8608 145:9<1895 2000 145 705 https://doi.org/10.1007/s007050070064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rustici G. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut NATIONALLICENCE 700 1- Accotto G. P. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut NATIONALLICENCE 700 1- Noris E. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut NATIONALLICENCE 700 1- Masenga V. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut NATIONALLICENCE 700 1- Luisoni E. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut NATIONALLICENCE 700 1- Milne R. G. Istituto di Fitovirologia Applicata del CNR, Torino, Italy, IT aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1895-1908 0304-8608 145:9<1895 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer