caa a22 4500 475765958 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070057 doi (NATIONALLICENCE)springer-10.1007/s007050070057 Apricot latent virus: a new species in the genus Foveavirus [Elektronische Daten] [L. G. Nemchinov, A. M. Shamloul, E. Z. Zemtchik, T. D. Verderevskaya, A. Hadidi] Summary.:  Extraction of viral double-stranded RNA from peach leaves infected with Apricot latent virus (ALV) followed by molecular cloning of synthesized cDNA and its sequencing, suggested that ALV is a new virus, whose coat protein (CP) coding region contains Apple stem pitting virus (ASPV)-related sequences. The sequenced portion of the ALV genome (1444 nt) includes the putative CP gene and the 3′ non-translated region. The 5′ portion of this fragment (1-651 nt) is highly distinct whereas the 3′ portion is 77% identical to the corresponding region of ASPV. Molecular hybridization experiments using a cRNA probe to ASPV with ALV-infected leaf tissue extracts also revealed that the genome of ALV contains nucleotide sequences related to that of ASPV. Western blots of tissue extracts indicated that ALV coat protein reacted with polyclonal antiserum against ASPV; however, the ALV CP differs in size from that of ASPV. ALV was graft-transmitted to several Prunus rootstocks. Based on the available sequence data, serological observations and bioassays we propose that ALV is a new species in the genus Foveavirus, typified by ASPV. ALV-specific PCR-primers and viral-specific cRNA probes developed in this investigation may be useful for detecting the virus and for studying its epidemiology and geographical distribution. Springer-Verlag/Wien, 2000 Nemchinov L. G. Vaccine and Therapeutic Development Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, U.S.A., US aut Shamloul A. M. Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, U.S.A., US aut Zemtchik E. Z. Research Institute of Horticulture, Chisinau, Moldova, MD aut Verderevskaya T. D. Research Institute of Horticulture, Chisinau, Moldova, MD aut Hadidi A. Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, U.S.A., US aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1801-1813 0304-8608 145:9<1801 2000 145 705 https://doi.org/10.1007/s007050070057 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070057 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nemchinov L. G. Vaccine and Therapeutic Development Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, U.S.A., US aut NATIONALLICENCE 700 1- Shamloul A. M. Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, U.S.A., US aut NATIONALLICENCE 700 1- Zemtchik E. Z. Research Institute of Horticulture, Chisinau, Moldova, MD aut NATIONALLICENCE 700 1- Verderevskaya T. D. Research Institute of Horticulture, Chisinau, Moldova, MD aut NATIONALLICENCE 700 1- Hadidi A. Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, U.S.A., US aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1801-1813 0304-8608 145:9<1801 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer