caa a22 4500 475765982 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070067 doi (NATIONALLICENCE)springer-10.1007/s007050070067 Molecular characterization and phylogenetic analysis of the Harrisina brillians granulovirus granulin gene [Elektronische Daten] [D. K. Bideshi, Y. Bigot, B. A. Federici] Summary.:  Well over 100 isolates of granulosis viruses (GVs), genus Granulovirus (family Baculoviridae), have been reported, all from lepidopterous insects. Three types of GVs are recognized, those of Type 1, which attack the fat body, Type 2, which attack most tissues, and Type 3, which attack only the midgut epithelium. To determine whether a correlation exists between tissue tropism and lepidopteran family phylogeny, the granulin gene of the Harrisina brillians (HbGV), a virus that attacks the midgut epithelium of H. brillians (family Zygaenidae) was cloned, sequenced, characterized, and compared with granulin genes of GVs that attack species of Tortricidae, Pieridae, and Noctuidae. The HbGV granulin gene encoded a peptide of 248 amino acids with a predicted Mr of 29.6 kDa, and shared a significant level of homology with other granulin (81-95% identical and 90-98% similar) and polyhedrin (49-58% identical and 62-72% similar) proteins. Phylogenetic analyses based on granulin and polyhedrin genes as well as on their 5′-untranslated sequences (5′-UTSs) indicated that HbGV was more closely related to GVs isolated from the tortricids, Cryptophlebia leucotreta (ClGV), Cydia pomonella (CpGV) and Choristoneura fumiferana (CfGV) than to other GVs and NPVs. This analysis provides preliminary evidence for a correlation between GV tissue tropism and the phylogeny of lepidopteran families, suggesting that GVs attacking species of Tortricidae and Zygaenidae are ancestral to those attacking species of the family Noctudiae. Springer-Verlag/Wien, 2000 Bideshi D. K. Interdepartment Graduate Programs in Genetics, University of California, Riverside, California, U.S.A., US aut Bigot Y. Institut de Recherche sur la Biologie de l'Insecte, Tours, France, FR aut Federici B. A. Interdepartment Graduate Programs in Genetics, University of California, Riverside, California, U.S.A., US aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1933-1945 0304-8608 145:9<1933 2000 145 705 https://doi.org/10.1007/s007050070067 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070067 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bideshi D. K. Interdepartment Graduate Programs in Genetics, University of California, Riverside, California, U.S.A., US aut NATIONALLICENCE 700 1- Bigot Y. Institut de Recherche sur la Biologie de l'Insecte, Tours, France, FR aut NATIONALLICENCE 700 1- Federici B. A. Interdepartment Graduate Programs in Genetics, University of California, Riverside, California, U.S.A., US aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1933-1945 0304-8608 145:9<1933 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer