caa a22 4500 475765990 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070070 doi (NATIONALLICENCE)springer-10.1007/s007050070070 Characterisation of two citrus apscaviroids isolated in Spain [Elektronische Daten] [X. Foissac, N. Duran-Vila] Summary.:  Sequence variability in the PCR amplified cDNAs from two citrus apscaviroid isolates CVd-Ia and CVd-IIId from Spain, was analysed. CVd-IIId sequence was shown to be identical to previously described CVd-III sequences and no important variability was encountered within the viroid population. Conversely, CVd-Ia displayed population heterogeneity as shown by SSCP analysis, Sal I restriction site polymorphism and sequences of 27 CVd-Ia cloned DNAs. The CVd-Ia genomic heterogeneity is characterised by two major subpopulations with the most divergent sequences, and by the presence of individual variants, making a sequence continuum between the two major groups. Most sequence variations are clustered in the left part of the viroid molecule. Springer-Verlag/Wien, 2000 Foissac X. Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain, ES aut Duran-Vila N. Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain, ES aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1975-1983 0304-8608 145:9<1975 2000 145 705 https://doi.org/10.1007/s007050070070 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070070 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Foissac X. Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain, ES aut NATIONALLICENCE 700 1- Duran-Vila N. Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain, ES aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1975-1983 0304-8608 145:9<1975 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer