caa a22 4500 475766016 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s007050070058 doi (NATIONALLICENCE)springer-10.1007/s007050070058 Presentation of antigenic sites from foot-and-mouth disease virus on the surface of baculovirus and in the membrane of infected cells [Elektronische Daten] [C. Tami, M. Farber, E. L. Palma, O. Taboga] Summary.:  We describe the construction of recombinant baculoviruses displaying on their surface and in the membrane of infected cells the small, immunodominant antigenic site (site A) or the large polyprotein (P1) coding for the four structural proteins of foot-and-mouth disease virus (FMDV). The coding sequences were inserted in the amino-terminus of gp64, the major glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). Following infection of insect cells with the recombinant baculoviruses, the cellular localization of the chimaeric proteins as well as their presence in the surface of extracellular viruses was assessed by immunofluorescence microscopy and Western blot. The antigenicity of the recombinant viruses was studied by competitive ELISAs, which showed that although both recombinant viruses were able to compete with FMDV-specific monoclonal antibodies (MAbs), their patterns of reactivity were different. The results suggest that this eukaryotic display system could be an alternative method of presentation of foreign antigens in a multimeric form as a new approach to biosynthetic vaccines. Springer-Verlag/Wien, 2000 Tami C. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut Farber M. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut Palma E. L. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut Taboga O. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut Archives of Virology Springer-Verlag 145/9(2000-09-01), 1815-1828 0304-8608 145:9<1815 2000 145 705 https://doi.org/10.1007/s007050070058 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070058 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tami C. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut NATIONALLICENCE 700 1- Farber M. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut NATIONALLICENCE 700 1- Palma E. L. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut NATIONALLICENCE 700 1- Taboga O. Instituto de Biotecnología, CICVyA, INTA, Castelar, Buenos Aires, Argentina, AR aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/9(2000-09-01), 1815-1828 0304-8608 145:9<1815 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer