caa a22 4500 475766040 CHVBK 20180406123605.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s007050070095 doi (NATIONALLICENCE)springer-10.1007/s007050070095 Genomic variability of equine herpesvirus-5 [Elektronische Daten] [M. Dunowska, S. A. Holloway, C. R. Wilks, J. Meers] Summary.:  Seventeen New Zealand isolates of equine herpesvirus 5 (EHV-5) were compared to the Australian prototype strain. PCR primers were designed to amplify EHV-5 glycoprotein B (gB) gene, and Restriction Fragment Length Polymorphism (RFLP) was used to detect differences between cloned PCR products. EHV-5 isolates from different horses showed a high degree of heterogeneity. However, EHV-5 isolates from individual horses remained homogeneous when examined over a period of time or isolated from different sites. A single EHV-5 gB RFLP profile was detected in isolates from each individual horse but one. Two or possibly three different genotypes of EHV-5 were detected in cultures inoculated with a nasal swab of this horse. The heterogeneity observed between EHV-5 isolates from different horses suggests that the use of RFLP may provide a useful epidemiological approach to gain more knowledge about the biology of EHV-5. Springer-Verlag/Wien, 2000 Dunowska M. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, NZ aut Holloway S. A. Centre for Equine Virology, The University of Melbourne, Parkville, Australia, AU aut Wilks C. R. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, NZ aut Meers J. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, NZ aut https://doi.org/10.1007/s007050070095 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070095 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dunowska M. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, NZ aut NATIONALLICENCE 700 1- Holloway S. A. Centre for Equine Virology, The University of Melbourne, Parkville, Australia, AU aut NATIONALLICENCE 700 1- Wilks C. R. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, NZ aut NATIONALLICENCE 700 1- Meers J. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand, NZ aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer