caa a22 4500 475766091 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s007050070100 doi (NATIONALLICENCE)springer-10.1007/s007050070100 Cross-reactive and major virus-specific epitopes are located at the N-terminal halves of the cylindrical inclusion proteins of turnip mosaic and zucchini yellow mosaic potyviruses [Elektronische Daten] [A. K. Kundu, K. Ohshima, N. Sako, H. Yaegashi] Summary.:  To investigate the antigenic nature of cylindrical inclusion proteins (CIPs) of the potyviruses Turnip mosaic virus (TuMV) and Zucchini yellow mosaic virus (ZYMV), monoclonal antibodies (MAbs) against the two CIPs were produced and epitopes on the CIPs were localized using Escherichia coli-expressed CIP fragments in Western blot analysis. All 23 MAbs against ZYMV CIP reacted only with ZYMV CIP. In contrast out of the 18 MAbs produced against TuMV CIP, 14 MAbs were TuMV CIP-specific while the remaining four MAbs cross-reacted with both CIPs. The four cross-reactive MAbs recognized two distinct epitopes in the N-terminal half of TuMV CIP corresponding to amino acid residues 103-119 and 224-237. Thirteen out of 14 TuMV CIP-specific MAbs recognized two distinct epitopes within residues 1-102 and 120-214, while the other one recognized an epitope within residues 301-644. On the other hand, 21 out of 23 ZYMV CIP-specific MAbs recognized epitopes within residues 1-118, while the remaining two recognized epitopes within residues 301-522. These results suggest that cross-reactive and major virus-specific epitopes are located at the N-terminal half of the respective CIPs. Springer-Verlag/Wien, 2000 Kundu A. K. Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga, Japan, JP aut Ohshima K. Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga, Japan, JP aut Sako N. Saga University, Saga, Japan, JP aut Yaegashi H. Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Saga, Japan, JP aut https://doi.org/10.1007/s007050070100 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070100 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kundu A. K. Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga, Japan, JP aut NATIONALLICENCE 700 1- Ohshima K. Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga, Japan, JP aut NATIONALLICENCE 700 1- Sako N. Saga University, Saga, Japan, JP aut NATIONALLICENCE 700 1- Yaegashi H. Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Saga, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer