caa a22 4500 475766199 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s007050070091 doi (NATIONALLICENCE)springer-10.1007/s007050070091 Ineffective control of murine cytomegalovirus by IE1-specific cytotoxic T lymphocytes during protracted infection in the lung [Elektronische Daten] [Q. J. Chen, H. Yoshida, H. Takimoto, T. Ninomiya, M. Ohtsu, G. Kimura, K. Nomoto] Summary.:  Interstitial pneumonia caused by cytomegalovirus (CMV) is a fatal disease in immunocompromised patients. In order to examine the defense mechanism against the virus in the lung, we employed an intratracheal infection model in susceptible mice. In mice infected intratracheally with murine CMV, a protracted infection was observed where infectious virus was detected up to 21 days of infection. During this prolonged infection, massive accumulation in the lung of CD8+ T cells with activated phenotypes occurred and these CD8+ T cells showed direct ex vivo cytolytic activity against target cells pulsed with the nonamer peptide derived from IE1 protein of the virus, which has been shown to be the dominant epitope recognized by most of virus-specific CTL. Moreover, adoptive transfer of in vitro induced IE1 peptide-specific CTL line showed no anti-virus effect in the lung, although they were effective in the spleen. Hence, there is reason to assume the IE1-specific CTL induced in vivo or in vitro plays limited roles during the prolonged infection in the lung. Springer-Verlag/Wien, 2000 Chen Q. J. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut Yoshida H. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut Takimoto H. Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan, JP aut Ninomiya T. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut Ohtsu M. Department of Virology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut Kimura G. Department of Virology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut Nomoto K. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut https://doi.org/10.1007/s007050070091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Q. J. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Yoshida H. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Takimoto H. Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan, JP aut NATIONALLICENCE 700 1- Ninomiya T. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Ohtsu M. Department of Virology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Kimura G. Department of Virology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Nomoto K. Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer