caa a22 4500 475766210 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070005 doi (NATIONALLICENCE)springer-10.1007/s007050070005 Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses [Elektronische Daten] [T. Hataya, K. Uchino, R. Arimoto, N. Suda, T. Sano, E. Shikata, I. Uyeda] Summary.:  The complete nucleotide sequence of the hop latent virus (HpLV) genome was determined. The viral RNA genome is 8,612 nucleotides long, excluding the poly(A) tail, and contains six open reading frames (ORFs), which encode putative proteins of 224-kDa (ORF 1), 25-kDa (ORF 2), 11-kDa (ORF 3), 7-kDa (ORF 4), 34-kDa (ORF 5), and 12-kDa (ORF 6). ORF 5 encodes the coat protein as demonstrated by N-terminal sequencing of three proteolytic peptides derived from the virus particle. The genome organization of HpLV is similar to that of other species in the genus Carlavirus, and the overall sequence of HpLV is more similar to that of Potato virus M than to sequences of other carlaviruses reported to date. The amino acid sequences of the putative methyltransferase, RNA helicase, and RNA-dependent RNA polymerase encoded in ORF 1 and an ‘accessory' helicase encoded in ORF 2 of the HpLV genome were compared with those of viruses in the ‘tymo' lineage: the genera Carlavirus, Potexvirus, Allexivirus, Foveavirus, Trichovirus, Capillovirus, Vitivirus, and Tymovirus. The phylogenetic relationships among the viruses in these genera are discussed. This is the first molecular characterization of a carlavirus infecting hop plants. Springer-Verlag/Wien, 2000 Hataya T. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut Uchino K. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut Arimoto R. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut Suda N. Hop Research Department, Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd., Hokkaido, Japan, JP aut Sano T. Department of Environmental Biology, Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan, JP aut Shikata E. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut Uyeda I. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut https://doi.org/10.1007/s007050070005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hataya T. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut NATIONALLICENCE 700 1- Uchino K. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut NATIONALLICENCE 700 1- Arimoto R. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut NATIONALLICENCE 700 1- Suda N. Hop Research Department, Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd., Hokkaido, Japan, JP aut NATIONALLICENCE 700 1- Sano T. Department of Environmental Biology, Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan, JP aut NATIONALLICENCE 700 1- Shikata E. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut NATIONALLICENCE 700 1- Uyeda I. Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer