caa a22 4500 475766237 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070013 doi (NATIONALLICENCE)springer-10.1007/s007050070013 Genetic composition and complexity of virus populations at tungro-endemic and outbreak rice sites [Elektronische Daten] [O. Azzam, M. Arboleda, K. M. L. Umadhay, J. B. de los Reyes, F. S. Cruz, A. Mackenzie, K. L. McNally] Summary.:  We have recently demonstrated the geographic isolation of rice tungro bacilliform virus (RTBV) populations in the tungro-endemic provinces of Isabela and North Cotabato, Philippines. In this study, we examined the genetic structure of the virus populations at the tungro-outbreak sites of Lanao del Norte, a province adjacent to North Cotabato. We also analyzed the virus populations at the tungro-endemic sites of Subang, Indonesia, and Dien Khanh, Vietnam. Total DNA extracts from 274 isolates were digested with EcoRV restriction enzyme and hybridized with a full-length probe of RTBV. In the total population, 22 EcoRV-restricted genome profiles (genotypes) were identified. Although overlapping genotypes could be observed, the outbreak sites of Lanao del Norte had a genotype combination distinct from that of Subang or Dien Khanh but a genotype combination similar to that identified earlier from North Cotabato, the adjacent endemic province. Sequence analysis of the intergenic region and part of the ORF1 RTBV genome from randomly selected genotypes confirms the geographic clustering of RTBV genotypes and, combined with restriction analysis, the results suggest a fragmented spatial distribution of RTBV local populations in the three countries. Because RTBV depends on rice tungro spherical virus (RTSV) for transmission, the population dynamics of both tungro viruses were then examined at the endemic and outbreak sites within the Philippines. The RTBV genotypes and the coat protein RTSV genotypes were used as indicators for virus diversity. A shift in population structure of both viruses was observed at the outbreak sites with a reduced RTBV but increased RTSV gene diversity. Springer-Verlag/Wien, 2000 Azzam O. International Rice Research Institute, Makati City, Philippines aut Arboleda M. International Rice Research Institute, Makati City, Philippines aut Umadhay K. M. L. International Rice Research Institute, Makati City, Philippines aut de los Reyes J. B. International Rice Research Institute, Makati City, Philippines aut Cruz F. S. International Rice Research Institute, Makati City, Philippines aut Mackenzie A. Research School of Biological Sciences, The Australian National University, Canberra, Australia, AU aut McNally K. L. International Rice Research Institute, Makati City, Philippines aut https://doi.org/10.1007/s007050070013 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070013 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Azzam O. International Rice Research Institute, Makati City, Philippines aut NATIONALLICENCE 700 1- Arboleda M. International Rice Research Institute, Makati City, Philippines aut NATIONALLICENCE 700 1- Umadhay K. M. L. International Rice Research Institute, Makati City, Philippines aut NATIONALLICENCE 700 1- de los Reyes J. B. International Rice Research Institute, Makati City, Philippines aut NATIONALLICENCE 700 1- Cruz F. S. International Rice Research Institute, Makati City, Philippines aut NATIONALLICENCE 700 1- Mackenzie A. Research School of Biological Sciences, The Australian National University, Canberra, Australia, AU aut NATIONALLICENCE 700 1- McNally K. L. International Rice Research Institute, Makati City, Philippines aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer