caa a22 4500 475766253 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070014 doi (NATIONALLICENCE)springer-10.1007/s007050070014 Evidence for two distinct subgroups of Alfalfa mosaic virus (AMV) from France and Italy and their relationships with other AMV strains [Elektronische Daten] [G. Parrella, C. Lanave, G. Marchoux, M. M. Finetti Sialer, A. Di Franco, D. Gallitelli] Summary.:  The nucleotide sequence of the putative coat protein open reading frame of seven previously uncharacterized AMV strains from Italy and France was determined and aligned with comparable sequences of other AMV strains (425 L, 425 M, YSMV, S, VRU, 15/64 and Da). The data set of AMV sequences was used to determine phylogenetic relationships by both a stochastic (stationary Markov model) and a deterministic method (maximum-parsimony) of analysis. The topology of the trees obtained with the two methods was essentially the same showing that all AMV strains clustered in two monophyletic groups. Close clustering of Italian strains in subgroup I and of French strains in subgroup II seems to suggests the effect of geographic distinctiveness of evolutionary dynamics of these AMV strains. This separation did not correlate with differences in host range or symptoms (necrotic or non necrotic) induced in tomato but rather it reflected variations in the amino acid sequence of their CP, which might be related to structural properties of virus particles. A simple and rapid procedure based on the reverse transcriptase-polymerase chain reaction (RT-PCR) followed by ezymatic digestion (RFLP) was developed to identify and classify AMV isolates into the two subgroups. The method applied to a number of other AMV isolates from Italy and France supported their division in two distinct subgroups. This RT-PCR RFLP method may be useful way to investigate the dynamics of AMV populations in nature. Springer-Verlag/Wien, 2000 Parrella G. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut Lanave C. Centro di Studio del CNR sui Mitocondri e Metabolismo Energetico, Bari, Italy, IT aut Marchoux G. INRA Station de Pathologie Vegetale, Montfavet, France, FR aut Finetti Sialer M. M. Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut Di Franco A. Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut Gallitelli D. Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut https://doi.org/10.1007/s007050070014 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070014 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Parrella G. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut NATIONALLICENCE 700 1- Lanave C. Centro di Studio del CNR sui Mitocondri e Metabolismo Energetico, Bari, Italy, IT aut NATIONALLICENCE 700 1- Marchoux G. INRA Station de Pathologie Vegetale, Montfavet, France, FR aut NATIONALLICENCE 700 1- Finetti Sialer M. M. Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut NATIONALLICENCE 700 1- Di Franco A. Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut NATIONALLICENCE 700 1- Gallitelli D. Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer