caa a22 4500 47576630X CHVBK 20180406123606.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070003 doi (NATIONALLICENCE)springer-10.1007/s007050070003 Isolation and characterization of simian immunodeficiency virus variants that are resistant to nonnucleoside reverse transcriptase inhibitors [Elektronische Daten] [Y. Isaka, S. Miki, S. Kawauchi, A. Suyama, H. Sugimoto, A. Adachi, M. Hayami, O. Yoshie, T. Fujiwara, A. Sato] Summary.:  Non-nucleoside reverse transcriptase inhibitors (NNRTIs) act quite specifically on human immunodeficiency virus type 1 (HIV-1). In general, they are not effective on human immunodeficiency virus type 2 (HIV-2) or simian immunodeficiency virus (SIV). Only SIV strains from African green monkeys are sensitive to several NNRTIs. Here we isolated NNRTI- and 3TC-resistant SIVagm variants. Viruses resistant to delavirdine contained V112I and M231I substitutions, while those resistant to 3TC contained a M185I substitution. These amino acids are highly conserved in HIV-1, HIV-2, SIVmac and SIVagm, and the M184I (M185I in SIVagm) substitution was observed in 3TC-resistant HIV-1 and SIVmac. The roles of the observed mutations in NNRTI-resistance of SIVagm and HIV-1 were further confirmed by site-directed mutagenesis. The present results have provided a new insight into the common mechanism of sensitivity of HIV-1 and SIVagm to NNRTIs. Springer-Verlag/Wien, 2000 Isaka Y. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Miki S. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Kawauchi S. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Suyama A. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Sugimoto H. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Adachi A. Department of Virology, Tokushima University, Tokushima, Japan, JP aut Hayami M. Institute of Virus Research, Kyoto University, Kyoto, Japan, JP aut Yoshie O. Department of Bacteriology, Kinki University School of Medicine, Osaka, Japan, JP aut Fujiwara T. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Sato A. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut https://doi.org/10.1007/s007050070003 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070003 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Isaka Y. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut NATIONALLICENCE 700 1- Miki S. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut NATIONALLICENCE 700 1- Kawauchi S. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut NATIONALLICENCE 700 1- Suyama A. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut NATIONALLICENCE 700 1- Sugimoto H. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut NATIONALLICENCE 700 1- Adachi A. Department of Virology, Tokushima University, Tokushima, Japan, JP aut NATIONALLICENCE 700 1- Hayami M. Institute of Virus Research, Kyoto University, Kyoto, Japan, JP aut NATIONALLICENCE 700 1- Yoshie O. Department of Bacteriology, Kinki University School of Medicine, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Fujiwara T. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut NATIONALLICENCE 700 1- Sato A. Discovery Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer