caa a22 4500 475766369 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070007 doi (NATIONALLICENCE)springer-10.1007/s007050070007 Genomic organization of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus [Elektronische Daten] [X. Chen, M. Li, X. Sun, B. M. Arif, Z. Hu, J. M. Vlak] Summary.:  The genome organization of the Helicoverpa armigera single-nucleo-capsid nucleopolyhedrovirus (HearNPV) was investigated and compared to that of other baculoviruses. A detailed physical map was constructed for the restriction enzymes BamHI, BglII, EcoRI, HindIII, KpnI, PstI, SacI and XhoI. The 130.1-kilobase pairs (kb) long viral genome was cloned as restriction fragments into a plasmid library, which covered 97.5% of the viral genome. From this plasmid library about 45 kb of dispersed sequence information was generated. Fifty-three putative open reading frames (ORFs) with homology to ORFs of other baculoviruses were identified and their location on the genome of HearNPV was determined. The arrangement of the ORFs along the HearNPV genome is significantly different from the multiple-nucleocapsid NPVs, AcMNPV, BmNPV and OpMNPV, which have a highly collinear genome, and the granulovirus XcGV. In contrast the genomes of HearNPV and SeMNPV are highly collinear. This close relatedness between an MNPV and an SNPV is supported by the phylogeny of specific genes of these two viruses and suggests that the morphotype (S or M) is only a taxonomic and not a phylogenetic denominator. The data presented here also give further support to the view that the gene distribution along baculovirus genomes can be used as a character to describe baculovirus relatedness independent of gene phylogeny. Five homologous regions (hrs) were located on the genome of HearNPV. This is also the first report of the existence of hrs in SNPVs and this observation suggests that hrs are a common feature of baculovirus genomes. Springer-Verlag/Wien, 2000 Chen X. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut Li M. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut Sun X. Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China, CN aut Arif B. M. Forest Pest Management Institute, Sault Ste. Marie, Ontario Canada, CA aut Hu Z. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut Vlak J. M. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut https://doi.org/10.1007/s007050070007 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen X. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Li M. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Sun X. Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China, CN aut NATIONALLICENCE 700 1- Arif B. M. Forest Pest Management Institute, Sault Ste. Marie, Ontario Canada, CA aut NATIONALLICENCE 700 1- Hu Z. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut NATIONALLICENCE 700 1- Vlak J. M. Laboratory of Virology, Wageningen University, Wageningen, The Netherlands, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer