caa a22 4500 475766407 CHVBK 20180406123606.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s007050050054 doi (NATIONALLICENCE)springer-10.1007/s007050050054 The nucleotide sequence and genome organization of Japanese iris necrotic ring virus , a new species in the genus Carmovirus [Elektronische Daten] [Y. Takemoto, T. Kanehira, M. Shinohara, S. Yamashita, T. Hibi] Summary.:  The genome of Japanese iris necrotic ring virus (JINRV) consists of a positive-sense ssRNA of 4014 nucleotides with six major open reading frames (ORFs). A 5′-non-coding region of 31 nucleotides precedes the first initiation codon. Like Carnation mottle virus (CarMV), the 5′-proximal three ORFs encode a 26 kDa protein (p26) and two readthrough proteins, i.e. an 85 kDa putative RNA replicase (p85) and a 99 kDa protein (p99). The central ORF encodes a small 8 kDa protein (p8). The 3′-proximal ORF encodes a 38 kDa capsid protein (p38). Another ORF encoding a 12 kDa protein (p12) overlaps the p99 ORF. JINRV RNA treated with bacterial alkaline phosphatase and tobacco acid pyrophosphatase could not be ligated to an oligoribonucleotide using T4 RNA ligase, indicating that the 5′ end of the viral RNA is uncapped. The 3′ end is not polyadenylated. Comparison of the genomic organization and the predicted amino acid sequences with those of other viruses confirmed that JINRV should be classified as a member of the genus Carmovirus, family Tombusviridae. 2000 Springer-Verlag/, Wien Takemoto Y. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan, JP aut Kanehira T. College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan, JP aut Shinohara M. College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan, JP aut Yamashita S. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan, JP aut Hibi T. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan, JP aut https://doi.org/10.1007/s007050050054 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050054 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takemoto Y. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Kanehira T. College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan, JP aut NATIONALLICENCE 700 1- Shinohara M. College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan, JP aut NATIONALLICENCE 700 1- Yamashita S. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Hibi T. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, The University of Tokyo, Tokyo, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer