caa a22 4500 47576644X CHVBK 20180406123607.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s007050050050 doi (NATIONALLICENCE)springer-10.1007/s007050050050 High prevalence of serum antibodies to Ras and type 16 E4 proteins of human papillomavirus in patients with precancerous lesions of the uterine cervix [Elektronische Daten] [A. Pedroza-Saavedra, A. Cruz, F. Esquivel, F. De La Torre, J. Berumen, P. Gariglio, L. Gutiérrez-X] Summary.:  Serum samples from 38 healthy women and 55 women with different types of cervical lesions were investigated for the presence of antibodies to Ras and against E4 and E7 proteins of human papillomavirus type 16 (HPV-16). Our results showed that anti-E7 antibodies were closely associated with cervical cancer (75%), as previously reported. Interestingly, E4 antibodies showed higher prevalence in condyloma lesions (79%; 11/14) than in cervical cancer (60%; 12/20). We also identified 11% (4/38) of healthy individuals as positive for E4 antibodies, which suggests an early immune recognition of this protein. Patients with condyloma and cervical intraepithelial neoplasia (CIN) also showed higher prevalences of Ras antibodies (∼40%) than cervical cancer patients (10%; 2/20). By sequencing part of the ras genes and using different Ras antigens, we showed that serum antibodies from patients were not directed to a Ras mutation, since wild-type cHa-Ras protein was recognized by these antibodies. In addition, patients positive for Ras antibodies (94%) were also positive for E4 antibodies, suggesting an association between these. The high prevalence of antibodies against Ras and E4 proteins in pre-malignant lesions opens the possibility of using both antibodies as early markers for potential cervical cancer patients. 2000 Springer-Verlag/, Wien Pedroza-Saavedra A. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México, MX aut Cruz A. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México, MX aut Esquivel F. Facultad de Medicina, Universidad Autónoma del Edo. de Morelos, Cuernavaca, Morelos, México, MX aut De La Torre F. Hospital "Adolfo López Mateos”, México D.F., México, MX aut Berumen J. Area de Biología Molecular, Escuela Militar de Graduados de Sanidad, México D.F., México, MX aut Gariglio P. Departamento de Genética y Biología Molecular, CINVESTAV-IPN y CICATA-IPN, México D.F., México, MX aut Gutiérrez-X L. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México, MX aut https://doi.org/10.1007/s007050050050 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050050 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pedroza-Saavedra A. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México, MX aut NATIONALLICENCE 700 1- Cruz A. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México, MX aut NATIONALLICENCE 700 1- Esquivel F. Facultad de Medicina, Universidad Autónoma del Edo. de Morelos, Cuernavaca, Morelos, México, MX aut NATIONALLICENCE 700 1- De La Torre F. Hospital "Adolfo López Mateos”, México D.F., México, MX aut NATIONALLICENCE 700 1- Berumen J. Area de Biología Molecular, Escuela Militar de Graduados de Sanidad, México D.F., México, MX aut NATIONALLICENCE 700 1- Gariglio P. Departamento de Genética y Biología Molecular, CINVESTAV-IPN y CICATA-IPN, México D.F., México, MX aut NATIONALLICENCE 700 1- Gutiérrez-X L. Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México, MX aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer