caa a22 4500 475766482 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s007050050043 doi (NATIONALLICENCE)springer-10.1007/s007050050043 Definition of an epitope on Japanese encephalitis virus (JEV) envelope protein recognized by JEV-specific murine CD8+ cytotoxic T lymphocytes [Elektronische Daten] [K. Takada, H. Masaki, E. Konishi, M. Takahashi, I. Kurane] Summary.:  We defined an epitope on the Japanese encephalitis virus (JEV) envelope (E) protein recognized by CD8+ cytotoxic T lymphocytes (CTLs). CTLs induced in JEV-infected BALB/c (H-2d) mice recognized E and/or premembrane (PrM) proteins, while CTLs in C57BL/6J (H-2b) and C3H/HeJ (H-2k) mice did not. JEV-specific CTLs had a phenotype of CD3+ CD4− CD8+. Twenty-four 9-amino acid (a.a.) peptides, which had binding motifs for H-2Kd, H-2Ld or H-2Dd, were synthesized according to the amino acid sequences of PrM and E proteins. CTLs induced by JEV infection recognized only the peptide K-3. Immunization of BALB/c mice with only a group of peptides including K-3 induced CTLs which recognized the homologous K-3 peptide, while immunization with other peptides did not. The peptide K-3 had a binding motif for H-2Kd. This is consistent with the finding that JEV-specific CTLs in BALB/c mice was H-2Kd-restricted. These results indicate that the epitope recognized by CTLs in BALB/c mice is located between a.a. 60 and 68 on the E protein, corresponding to an a.a. sequence of CYHASVTDI. 2000 Springer-Verlag/, Wien Takada K. Department of Neurology, Kinki University School of Medicine, Osakasayama, Japan, JP aut Masaki H. Department of Microbiology, Kinki University School of Medicine, Osakasayama, Japan, JP aut Konishi E. Department of Health Sciences, Kobe University School of Medicine, Kobe, Japan, JP aut Takahashi M. Department of Neurology, Kinki University School of Medicine, Osakasayama, Japan, JP aut Kurane I. Department of Microbiology, Kinki University School of Medicine, Osakasayama, Japan, JP aut https://doi.org/10.1007/s007050050043 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050043 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takada K. Department of Neurology, Kinki University School of Medicine, Osakasayama, Japan, JP aut NATIONALLICENCE 700 1- Masaki H. Department of Microbiology, Kinki University School of Medicine, Osakasayama, Japan, JP aut NATIONALLICENCE 700 1- Konishi E. Department of Health Sciences, Kobe University School of Medicine, Kobe, Japan, JP aut NATIONALLICENCE 700 1- Takahashi M. Department of Neurology, Kinki University School of Medicine, Osakasayama, Japan, JP aut NATIONALLICENCE 700 1- Kurane I. Department of Microbiology, Kinki University School of Medicine, Osakasayama, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer