caa a22 4500 475766512 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s007050050046 doi (NATIONALLICENCE)springer-10.1007/s007050050046 Grapevine fleck virus-like viruses in Vitis [Elektronische Daten] [S. Sabanadzovic, N. Abou-Ghanem, M. A. Castellano, M. Digiaro, G. P. Martelli] Summary.:  Two sets of degenerate primers for the specific amplification of 572-575 nt and 386 nt segments of the methyltransferase and RNA-dependent RNA polymerase cistrons of members of the genera Tymovirus and Marafivirus and of the unassigned virus Grapevine fleck virus (GFkV) were designed on the basis of available sequences. These primers were used for amplifying and subsequent cloning and sequencing part of the open reading frame 1 of the genome of GFkV, Grapevine asteroid mosaic-associated virus (GAMaV) and of another previously unreported virus, for which the name Grapevine red globe virus (GRGV) is proposed. Computer-assisted analysis of the amplified genome portions showed that the three grapevine viruses are phylogenetically related with one another and with sequenced tymoviruses and marafiviruses. The relationships with tymoviruses was confirmed by the type of ultrastructural modifications induced in the host cells. RdRp-specific degenerate primers were successfully used for the aspecific detection of the three viruses in crude grapevine sap extracts. Specific virus identification was obtained with RT-PCR using antisense virus-specific primers. 2000 Springer-Verlag/, Wien Sabanadzovic S. Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy, IT aut Abou-Ghanem N. Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy, IT aut Castellano M. A. Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut Digiaro M. Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy, IT aut Martelli G. P. Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut https://doi.org/10.1007/s007050050046 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050046 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sabanadzovic S. Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy, IT aut NATIONALLICENCE 700 1- Abou-Ghanem N. Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy, IT aut NATIONALLICENCE 700 1- Castellano M. A. Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut NATIONALLICENCE 700 1- Digiaro M. Istituto Agronomico Mediterraneo, Valenzano, Bari, Italy, IT aut NATIONALLICENCE 700 1- Martelli G. P. Dipartimento di Protezione delle Piante, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer