caa a22 4500 475766571 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070038 doi (NATIONALLICENCE)springer-10.1007/s007050070038 A vhs-like activity of bovine herpesvirus-1 [Elektronische Daten] [S. Hinkley, A. P. N. Ambagala, C. J. Jones, S. Srikumaran] Summary.:  Bovine herpesvirus 1 (BHV-1) is a major pathogen of cattle, causing significant disease including immunosuppression in infected animals. In vitro, the surface expression of major histocompatibility complex (MHC) class I molecules, crucial for an appropriate anti-viral immune response of the host, is down-regulated by BHV-1 infection. Northern blot analyses revealed that the mRNAs for MHC class I and class II molecules were significantly down-regulated in BHV-1 infected cells, starting as early as 2 h after infection. Furthermore, mRNA expression of the two house keeping genes actin and glyceraldehyde-6-phosphate dehydrogenase (GAPDH) was also repressed after infection. This BHV-1 induced effect on cellular metabolism resembled the virion host shutoff (vhs) activity of herpes simplex virus (HSV). Similar to the HSV vhs activity, the putative BHV-1 vhs activity was not abrogated in cells infected in the presence of actinomycin D (ActD) which suggested that no viral gene expression is required for the vhs function and the putative vhs protein is associated with the virion. Sequence comparison indicated a BHV-1 open reading frame having a 60% similarity to the HSV vhs sequence. This putative BHV-1 open reading frame contained the four conserved regions of the alphaherpesvirus vhs protein. Since an HSV vhs-mutant exhibited less virulence and good immunogenicity, we suggest that a BHV-1 vhs− mutant may hold promising potential as a candidate vaccine. Springer-Verlag/Wien, 2000 Hinkley S. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut Ambagala A. P. N. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut Jones C. J. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut Srikumaran S. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut https://doi.org/10.1007/s007050070038 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070038 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hinkley S. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Ambagala A. P. N. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Jones C. J. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut NATIONALLICENCE 700 1- Srikumaran S. Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer