caa a22 4500 47576658X CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070036 doi (NATIONALLICENCE)springer-10.1007/s007050070036 Seasonal change of infective state of marine birnavirus in Japanese pearl oyster Pinctada fucata [Elektronische Daten] [S.-I. Kitamura, S.-J. Jung, S. Suzuki] Summary.:  This study examines the seasonal occurrence and infective state of marine birnavirus (MABV) in cultured Japanese pearl oyster (Pinctada fucata). Planted oysters were sampled monthly in 1997 and 1998. To detect MABV in the oysters, PCR and virus isolation were carried out. Also, the indirect fluorescent antibody technique (IFAT) was performed to know the organs expressing viral antigens. The detection rate of the MABV genome by PCR was low during July to October, but increased after November. This virus was isolated only after October, with a 10-40% isolation rate. Results of the IFAT showed that the specific fluorescence was observed in hemocytes in September. Fluorescence in hemocytes decreased in January, but increased in liver parenchymal cells. These results suggest that MABV persistently infected hemocytes in summer with a small amount of genome and protein, and then the virus spread in winter into the parenchymal cells. Springer-Verlag/Wien, 2000 Kitamura S.-I Department of Aquaculture, Kochi University, Kochi, Japan, JP aut Jung S.-J Department of Aquaculture, Kochi University, Kochi, Japan, JP aut Suzuki S. Department of Aquaculture, Kochi University, Kochi, Japan, JP aut https://doi.org/10.1007/s007050070036 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070036 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kitamura S.-I Department of Aquaculture, Kochi University, Kochi, Japan, JP aut NATIONALLICENCE 700 1- Jung S.-J Department of Aquaculture, Kochi University, Kochi, Japan, JP aut NATIONALLICENCE 700 1- Suzuki S. Department of Aquaculture, Kochi University, Kochi, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer