caa a22 4500 475766601 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070045 doi (NATIONALLICENCE)springer-10.1007/s007050070045 Synthesis, subcellular localization and VP16 interaction of the herpes simplex virus type 2 UL46 gene product [Elektronische Daten] [K. Kato, T. Daikoku, F. Goshima, H. Kume, K. Yamaki, Y. Nishiyama] Summary.:  We developed a rabbit polyclonal antiserum reactive against a recombinant 6x His-UL46 fusion protein expressed in*Escherichia coli, and using this antiserum identified the UL46 gene product of herpes simplex virus type 2 (HSV-2) to be phosphoproteins with apparent molecular masses of 82-, 84-, and 86-kDa in infected Vero cells. The UL46 protein was produced in the late phase of infection in a manner highly dependent on viral DNA synthesis, and was mainly distributed at the edge of the nucleus in the cytoplasm. Although its kinetics of production and its progress of distribution were different from those of the major tegument protein VP16 (the UL48 gene product or α-trans-inducing factor (αTIF)), most of the UL46 protein colocalized with VP16 in the late phase of infection, and copurified with it in column chromatography. Moreover, our data showed that the HSV-2 UL46 protein, when coexpressed with VP16, enhanced α4 promotor-regulated gene expression in a transient luciferase reporter assay, while the expression of the UL46 protein alone suppressed it. Springer-Verlag/Wien, 2000 Kato K. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut Daikoku T. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut Goshima F. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut Kume H. Second Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan, JP aut Yamaki K. Second Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan, JP aut Nishiyama Y. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut https://doi.org/10.1007/s007050070045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato K. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut NATIONALLICENCE 700 1- Daikoku T. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut NATIONALLICENCE 700 1- Goshima F. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut NATIONALLICENCE 700 1- Kume H. Second Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan, JP aut NATIONALLICENCE 700 1- Yamaki K. Second Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan, JP aut NATIONALLICENCE 700 1- Nishiyama Y. Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer