caa a22 4500 47576661X CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070048 doi (NATIONALLICENCE)springer-10.1007/s007050070048 Human papillomavirus type 16 E5 protein localizes to the Golgi apparatus but does not grossly affect cellular glycosylation [Elektronische Daten] [C. Oetke, E. Auvinen, M. Pawlita, A. Alonso] Summary.:  The E5 protein of papillomaviruses is a strongly hydrophobic membrane protein that can associate with the 16 kDa protein subunit of the vacuolar proton ATPase in endosomes and the Golgi apparatus resulting in raise of intraorganelle pH. We demonstrate that E5 of human papillomavirus type 16 (HPV16) when transfected into human keratinocytes localizes to the Golgi. Using FACS analysis and western blotting with a variety of lectins as well as analysing the sialylation status of a specific cell surface glycoprotein CD95 (APO-1/Fas), we show that HPV16 E5 does not grossly affect cellular glycosylation, a main Golgi function. Springer-Verlag/Wien, 2000 Oetke C. Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany, DE aut Auvinen E. Department of Virology, Haartman Institute, University of Helsinki, Finland, FI aut Pawlita M. Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany, DE aut Alonso A. Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany, DE aut https://doi.org/10.1007/s007050070048 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070048 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oetke C. Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany, DE aut NATIONALLICENCE 700 1- Auvinen E. Department of Virology, Haartman Institute, University of Helsinki, Finland, FI aut NATIONALLICENCE 700 1- Pawlita M. Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany, DE aut NATIONALLICENCE 700 1- Alonso A. Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer