caa a22 4500 475766652 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070041 doi (NATIONALLICENCE)springer-10.1007/s007050070041 A single point mutation in HIV-1 V3 loop alters the immunogenic properties of rgp120 [Elektronische Daten] [S.-K. Lee, G. A. Pestano, J. Riley, A. S. Hasan, M. Pezzano, M. Samms, K. J. Park, J. Guyden, W. M. O. Boto] Summary.:  The results of the study presented in this report show that clones of env derived from genetically divergent HIV-1 field isolates fall into two major subsets based on the predicted secondary structure of the V3 region in gp120. One subset exemplified by the clones A-UG06c, B-RT3.12 and C-UG045 is predicted to assume a β-turn conformation in the V3 loop and comprises the GPG residues. The other subset exemplified by the clones D-UG23c and D-UG042 (G ) are deficient in the expression of the β-turn in the loop. Since secondary conformations are highly likely to confer antigenic properties in a protein backbone at least for B cells, we have used nucleic acid immunization to test the effect of the β-turn deficiency on the immunogenic potential of rgp120 encoded in these field isolates. As hypothesized, inoculation of BALB/c mice with the env plasmid encoding the β-turn expressing rgp120 molecules resulted in the development of a vigorous antibody response to the homologous V3 loop peptides. In contrast, immunization with an rgp120 clone deficient in the β-turn in the V3 loop showed no evidence of antibody development to the V3 loop. Instead, the latter clones triggered T cell proliferative responses and markedly increased the level of IL-2 and IFN-γ production by T cells. Significantly, reconstitution of the β-turn conformation by site-directed mutagenesis of a single V3 loop residue yielded rgp120 molecules which restored antibody production while diminishing the cell-mediated immune (CMI) responses to the V3 residue. These observations demonstrate the marked impact of a single amino acid substitution on the immunogenic properties of V3 region in gp120 encoded by divergent HIV-1 field isolates. Springer-Verlag/Wien, 2000 Lee S.-K Department of Biology, The City College of New York, New York, U.S.A., US aut Pestano G. A. Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, U.S.A., US aut Riley J. Department of Biology, The City College of New York, New York, U.S.A., US aut Hasan A. S. Department of Biology, The City College of New York, New York, U.S.A., US aut Pezzano M. Department of Biology, The City College of New York, New York, U.S.A., US aut Samms M. Department of Biology, The City College of New York, New York, U.S.A., US aut Park K. J. Department of Biology, The City College of New York, New York, U.S.A., US aut Guyden J. Department of Biology, The City College of New York, New York, U.S.A., US aut Boto W. M. O. Department of Biology, The City College of New York, New York, U.S.A., US aut https://doi.org/10.1007/s007050070041 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070041 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee S.-K Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Pestano G. A. Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, U.S.A., US aut NATIONALLICENCE 700 1- Riley J. Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Hasan A. S. Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Pezzano M. Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Samms M. Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Park K. J. Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Guyden J. Department of Biology, The City College of New York, New York, U.S.A., US aut NATIONALLICENCE 700 1- Boto W. M. O. Department of Biology, The City College of New York, New York, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer