caa a22 4500 475766679 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070042 doi (NATIONALLICENCE)springer-10.1007/s007050070042 A population of variants of a viroid closely related to citrus viroid-I in citrus plants [Elektronische Daten] [Takao Ito, Hiroyuki Ieki, Katsumi Ozaki] Summary.:  Sequencing analyses showed a population of variants consisting of 325-330 nucleotides (nt) of a viroid closely related to citrus viroid (CVd)-I in citrus plants. These variants, for which we propose the tentative acronym CVd-I-LSS (low sequence similarity), have only 82-85% sequence similarities to CVd-I variants. A phylogenetic tree showed that the CVd-I-LSS variants formed an individual cluster that was distinct from that of the CVd-I variants, but no intermediate variants were found which could continuously connect the population of CVd-I-LSS variants with that of CVd-I variants. Citrons (Citrus medica L.) inoculated with the CVd-I-LSS seemed to show not only moderate leaf bending like citrons infected with CVd-I but also slightly more severe epinasty than citrons with CVd-I. Other biological properties of CVd-I-LSS, such as the host range, will need to be determined in order to clarify whether CVd-I-LSS is a new viroid species or a distinct strain of CVd-I. Most of the nucleotide changes between the CVd-I-LSS and CVd-I variants were found at complementary positions of the upper and lower strands within the putative pathogenic, variable, and right terminal domains, as well as in the region surrounding the central conserved region of the predicted secondary structure of CVd-I-LSS. Springer-Verlag/Wien, 2000 Ito Takao Department of Citriculture, National Institute of Fruit Tree Science, Kuchinotsu, Nagasaki, Japan, JP aut Ieki Hiroyuki Persimmon and Grape Research Center, National Institute of Fruit Tree Science, Akitsu, Hiroshima, Japan, JP aut Ozaki Katsumi Faculty of Horticulture, Minami Kyushu University, Takanabe, Miyazaki, Japan, JP aut https://doi.org/10.1007/s007050070042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ito Takao Department of Citriculture, National Institute of Fruit Tree Science, Kuchinotsu, Nagasaki, Japan, JP aut NATIONALLICENCE 700 1- Ieki Hiroyuki Persimmon and Grape Research Center, National Institute of Fruit Tree Science, Akitsu, Hiroshima, Japan, JP aut NATIONALLICENCE 700 1- Ozaki Katsumi Faculty of Horticulture, Minami Kyushu University, Takanabe, Miyazaki, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer