caa a22 4500 475766695 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070049 doi (NATIONALLICENCE)springer-10.1007/s007050070049 Pseudorabies virus induces apoptosis in tissue culture cells [Elektronische Daten] [A. K. Cheung, Z. Chen, Z. Sun, D. McCullough] Summary.:  The process of cell death as a result of exposure to pseudorabies virus (PRV) in cultured cells was examined and specific features characteristic of apoptosis were observed. At early times of infection, externalization of membrane phospholipid phophatidylserine was detected by flow cytometry analysis. During the infection process, caspase 3-like protease activity was induced and the activity increased in a time dependent manner. Cellular DNA degradation was demonstrated by agarose gel electrophoresis. Morphologic changes of the nucleus that included chromatin condensation and margination to the periphery of the nucleus were evident in electron microscopy analysis. These biochemical and morphologic changes demonstrated that, during PRV replication, the host cell was induced to undergo apoptosis. Springer-Verlag/Wien, 2000 Cheung A. K. Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, U.S.A., US aut Chen Z. Institute of Microbiology and Epidemiology, Beijing, China, CN aut Sun Z. Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China, CN aut McCullough D. Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, U.S.A., US aut https://doi.org/10.1007/s007050070049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheung A. K. Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, U.S.A., US aut NATIONALLICENCE 700 1- Chen Z. Institute of Microbiology and Epidemiology, Beijing, China, CN aut NATIONALLICENCE 700 1- Sun Z. Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China, CN aut NATIONALLICENCE 700 1- McCullough D. Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer