caa a22 4500 475766709 CHVBK 20180406123607.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s007050070039 doi (NATIONALLICENCE)springer-10.1007/s007050070039 Glycoprotein C of bovine herpesvirus 5 (BHV-5) confers a distinct heparin-binding phenotype to BHV-1 [Elektronische Daten] [A. Liman, M. Engels, G. Meyer, M. Ackermann] Summary.:  Bovine herpesvirus type 1 (BHV-1) causes respiratory and genital diseases in cattle, whereas the closely related BHV-5 can induce severe meningoencephalitis in calves. To characterize BHV-5 glycoprotein C (gC5) within the backbone of BHV-1, three consecutive recombinant viruses were constructed: A deletion mutant (rBHV-1ΔgC blue) with gC1 replaced by the lacZ gene, an exchange mutant (rBHV-1gC5) with the lacZ of BHV-1ΔgC blue exchanged by gC5, and a rescue mutant (rescue BHV-1) from rBHV-1gC5 with an additional XbaI site in gC1. The recombinant and wildtype viruses were characterized on MDBK cells. Although no significant differences were observed in growth behaviour and entry kinetics, rBHV-1gC5 showed a distinct phenotype in a heparin blocking assay. The gC5 was able to transfer the heparin binding phenotype of BHV-5 to BHV-1. This indicates that gC1 and gC5 differ in their receptor binding qualities, which might modulate the ability of the viruses to spread within the central nervous system. Springer-Verlag/Wien, 2000 Liman A. Institute of Virology, Veterinary Medical Faculty, University of Zürich, Switzerland, CH aut Engels M. Institute of Virology, Veterinary Medical Faculty, University of Zürich, Switzerland, CH aut Meyer G. Veterinary Virology, University of Liège, Belgium, BE aut Ackermann M. Institute of Virology, Veterinary Medical Faculty, University of Zürich, Switzerland, CH aut https://doi.org/10.1007/s007050070039 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070039 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liman A. Institute of Virology, Veterinary Medical Faculty, University of Zürich, Switzerland, CH aut NATIONALLICENCE 700 1- Engels M. Institute of Virology, Veterinary Medical Faculty, University of Zürich, Switzerland, CH aut NATIONALLICENCE 700 1- Meyer G. Veterinary Virology, University of Liège, Belgium, BE aut NATIONALLICENCE 700 1- Ackermann M. Institute of Virology, Veterinary Medical Faculty, University of Zürich, Switzerland, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer