caa a22 4500 475766792 CHVBK 20180406123608.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s007050050667 doi (NATIONALLICENCE)springer-10.1007/s007050050667 Temporal and subcellular localization of infectious pancreatic necrosis virus structural proteins [Elektronische Daten] [J. C. Espinoza, A. Hjalmarsson, E. Everitt, J. Kuznar] Summary.:  The temporal subcellular localization of the structural proteins VP2 and VP3 of infectious pancreatic necrosis virus was analyzed with monoclonal antibodies conjugated with fluorochromes. Early in the infection both proteins were colocalized in the cytosol, at later times, VP2 was visualized as inclusion bodies around the nuclei of the cells and, sometimes, it was found in elongated tubular structures that might correspond to the type I tubules seen in cells infected with another Birnavirus. As VP2 is a glycosylated protein we determined its subcellular localization compared with that of ER and Golgi probes. These results suggest that VP2 is glycosylated freely in the cytoplasm. 2000 Springer-Verlag/, Wien Espinoza J. C. Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad de Valparaíso, Chile, CL aut Hjalmarsson A. Department of Microbiology, Lund University, Sweden, SE aut Everitt E. Department of Microbiology, Lund University, Sweden, SE aut Kuznar J. Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad de Valparaíso, Chile, CL aut https://doi.org/10.1007/s007050050667 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050667 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Espinoza J. C. Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad de Valparaíso, Chile, CL aut NATIONALLICENCE 700 1- Hjalmarsson A. Department of Microbiology, Lund University, Sweden, SE aut NATIONALLICENCE 700 1- Everitt E. Department of Microbiology, Lund University, Sweden, SE aut NATIONALLICENCE 700 1- Kuznar J. Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad de Valparaíso, Chile, CL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer