caa a22 4500 475766814 CHVBK 20180406123608.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s007050050672 doi (NATIONALLICENCE)springer-10.1007/s007050050672 pUL25 immunolocalization in human cytomegalovirus-infected and gene-transfected cells [Elektronische Daten] [N. Zini, S. Santi, M. Riccio, M. P. Landini, M. C. Battista, N. M. Maraldi] Summary.:  By means of confocal and electron microscope immunocytochemistry we have studied the localization of a recently described structural protein (pUL25) of human cytomegalovirus, in both infected cells and in cells transiently transfected with UL25. pUL25 localization in infected cells was observed in typical cytoplasmic structures characterized by a very electrondense texture previously reported to accumulate other tegument proteins. At the virion level pUL25 seems to localize at the interface between the tegument and the capsid of both intracytoplasmic and extracellular virions. In UL-25-transfected cells, pUL25 has been found in characteristic para-crystalline cytoplasmic aggregates, suggesting its intrinsic ability to aggregate in a regular subunit pattern. 2000 Springer-Verlag/, Wien Zini N. Institute of Normal and Pathologic Cytomorphology, C.N.R., c/o I.O.R., Bologna, Italy, IT aut Santi S. Institute of Normal and Pathologic Cytomorphology, C.N.R., c/o I.O.R., Bologna, Italy, IT aut Riccio M. Laboratory of Cellular Biology and Electron Microscopy, I.O.R., Bologna, Italy, IT aut Landini M. P. Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Bologna, Italy, IT aut Battista M. C. Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Bologna, Italy, IT aut Maraldi N. M. Institute of Normal and Pathologic Cytomorphology, C.N.R., c/o I.O.R., Bologna, Italy, IT aut https://doi.org/10.1007/s007050050672 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050672 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zini N. Institute of Normal and Pathologic Cytomorphology, C.N.R., c/o I.O.R., Bologna, Italy, IT aut NATIONALLICENCE 700 1- Santi S. Institute of Normal and Pathologic Cytomorphology, C.N.R., c/o I.O.R., Bologna, Italy, IT aut NATIONALLICENCE 700 1- Riccio M. Laboratory of Cellular Biology and Electron Microscopy, I.O.R., Bologna, Italy, IT aut NATIONALLICENCE 700 1- Landini M. P. Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Bologna, Italy, IT aut NATIONALLICENCE 700 1- Battista M. C. Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Bologna, Italy, IT aut NATIONALLICENCE 700 1- Maraldi N. M. Institute of Normal and Pathologic Cytomorphology, C.N.R., c/o I.O.R., Bologna, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer