caa a22 4500 475766849 CHVBK 20180406123608.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s007050050668 doi (NATIONALLICENCE)springer-10.1007/s007050050668 The presence of cross-reactive antibodies to rabbit haemorrhagic disease virus in Australian wild rabbits prior to the escape of virus from quarantine [Elektronische Daten] [H. S. Nagesha, K. A. McColl, B. J. Collins, C. J. Morrissy, L. -F. Wang, H. A. Westbury] Summary.:  Sera collected from Australian wild rabbits prior to the escape of rabbit haemorrhagic disease virus (RHDV) from Wardang Island were examined for RHDV antibodies using purified recombinant capsid protein VP60 expressed from baculovirus. A VP60-based indirect ELISA showed that 196 of 392 wild rabbit sera reacted (OD450>0.15) with VP60. Twenty sera (OD450 ranging from 0.15-2.47), randomly chosen from the 196 positive sera, recognized the 64 kDa VP60 in Western blot analysis, indicating that the reactivity detected in ELISA is indeed specific to the VP60 antigen. In a separate study, sera of 23 rabbits from an RHD-free area after the escape of RHDV were tested by ELISA and 21 of the 23 rabbits were found to be positive. When these rabbits were challenged with a lethal dose of RHDV, 11 out of the 23 rabbits survived. The presence of RHDV-protective antibodies in some of these rabbits suggested that they had been exposed to a pre-existing non-virulent rabbit calicivirus closely related to RHDV. These results highlight the need to study the prevalence of, and to characterize, this viral agent in order to effectively control rabbit populations in Australia and New Zealand. 2000 Springer-Verlag/, Wien Nagesha H. S. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut McColl K. A. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut Collins B. J. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut Morrissy C. J. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut Wang L. -F CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut Westbury H. A. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut https://doi.org/10.1007/s007050050668 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050668 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nagesha H. S. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- McColl K. A. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Collins B. J. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Morrissy C. J. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Wang L. -F CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Westbury H. A. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer