caa a22 4500 47576689X CHVBK 20180406123608.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s007050050671 doi (NATIONALLICENCE)springer-10.1007/s007050050671 Nucleotide sequence and synthesis of infectious RNA from cloned cDNA of broad bean wilt virus 2 RNA 2 [Elektronische Daten] [T. Kuroda, A. Okumura, I. Takeda, Y. Miura, K. Suzuki] Summary.:  The complete nucleotide sequence of broad bean wilt virus -2 (BBWV-2) RNA 2 was determined. The sequence is 3593 nucleotides in length, and contains a single open reading frame which has the potential to encode a 119 kDa polyprotein. Direct amino acid sequencing of the virus coat proteins suggests that the polyprotein is processed to produce three proteins, 52 kDa, 44 kDa and 22 kDa in size. A comparison of several fabaviruses in terms of nucleotide sequence and amino acid sequences showed that BBWV-2 isolates display high sequence identity. A full-length cDNA constructed under the control of the T7 promoter could be successfully transcribed to produce biologically active RNA. This is the first report on generating infectious transcripts of BBWV-2 RNA 2. 2000 Springer-Verlag/, Wien Kuroda T. Iwate Biotechnology Research Center, Kitakami, Iwate, Japan, JP aut Okumura A. Iwate Biotechnology Research Center, Kitakami, Iwate, Japan, JP aut Takeda I. aut Miura Y. aut Suzuki K. aut https://doi.org/10.1007/s007050050671 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050671 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kuroda T. Iwate Biotechnology Research Center, Kitakami, Iwate, Japan, JP aut NATIONALLICENCE 700 1- Okumura A. Iwate Biotechnology Research Center, Kitakami, Iwate, Japan, JP aut NATIONALLICENCE 700 1- Takeda I. aut NATIONALLICENCE 700 1- Miura Y. aut NATIONALLICENCE 700 1- Suzuki K. aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer