caa a22 4500 475766903 CHVBK 20180406123608.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s007050070082 doi (NATIONALLICENCE)springer-10.1007/s007050070082 The viral RNA 3′- and 5′-end structure and mRNA transcription of infectious salmon anaemia virus resemble those of influenza viruses [Elektronische Daten] [T. Sandvik, E. Rimstad, S. Mjaaland] Summary.:  The nucleotide sequences of the termini of two of the genomic segments of the negative strand RNA virus infectious salmon anaemia virus (ISAV) were determined. The sequence of the terminal 9 nucleotides at both ends of the viral RNAs was identical, and showed distinctive sequence homology with the conserved terminal sequences found in the orthomyxoviruses. For both ISAV genomic segments a computer-based secondary structure modelling indicated that the terminal 21-24 nucleotides were able to form self-complementary panhandle structures. Comparison with ISAV-derived mRNA sequences showed that ISAV mRNAs have heterogeneous 5′-ends, and are polyadenylated from a signal sequence 13-14 nucleotides downstream of the 5′-end terminus of the vRNA. Furthermore, the in vitro replication of ISAV was hindered by the RNA polymerase II inhibitor α-amanitin. These findings indicate that the mechanisms for replication of ISAV are similar to those of the orthomyxoviruses, and add to the previously reported structural similarities between ISAV and the orthomyxoviruses. Springer-Verlag/Wien, 2000 Sandvik T. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway, NO aut Rimstad E. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway, NO aut Mjaaland S. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway, NO aut https://doi.org/10.1007/s007050070082 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070082 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sandvik T. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway, NO aut NATIONALLICENCE 700 1- Rimstad E. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway, NO aut NATIONALLICENCE 700 1- Mjaaland S. Department of Pharmacology, Microbiology and Food Hygiene, The Norwegian School of Veterinary Science, Oslo, Norway, NO aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer